[不同微生物腹腔感染大鼠尿液蛋白质组的比较]

[Comparison of urine proteome in rats intraperitoneally infected by different microorganisms].

作者信息

Meng Wenshu, Zhang Yameng, Hua Yuanrui, Jiang Yijin, Kong Jiawen, Gao Youhe

机构信息

Gene Engineering Drug and Biotechnology Beijing Key Laboratory, Department of Biochemistry and Molecular Biology, Beijing Normal University, Beijing 100875, China.

出版信息

Sheng Wu Gong Cheng Xue Bao. 2021 Jan 25;37(1):276-289. doi: 10.13345/j.cjb.200214.

DOI:10.13345/j.cjb.200214

PMID:33501808

Abstract

Different microorganisms can cause intraperitoneal infection. This study was to distinguish different microbial infections by urine analysis. Rats were intraperitoneally injected with Escherichia coli, Staphylococcus aureus, and Candida albicans, separately. Urine samples were collected from rats at 0, 12, 36 and 72 h after infection. Urinary proteins were profiled using liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). Compared with the control (without infection), a total of 69 differential proteins were identified in rats injected with E. coli. A total of 31 differences proteins were identified in rats injected with S. aureus. A total of 38 differential proteins were identified in rats injected with C. albicans. Urine proteome was different when rats were infected by different microorganisms, suggesting that urine may have the potential for differential diagnosis of different intraperitoneal infections.

摘要

不同的微生物可引起腹腔感染。本研究旨在通过尿液分析区分不同的微生物感染。将大鼠分别腹腔注射大肠杆菌、金黄色葡萄球菌和白色念珠菌。在感染后0、12、36和72小时从大鼠收集尿液样本。使用液相色谱-串联质谱（LC-MS/MS）对尿蛋白进行分析。与对照组（未感染）相比，注射大肠杆菌的大鼠共鉴定出69种差异蛋白。注射金黄色葡萄球菌的大鼠共鉴定出31种差异蛋白。注射白色念珠菌的大鼠共鉴定出38种差异蛋白。当大鼠受到不同微生物感染时，尿蛋白质组不同，这表明尿液可能具有鉴别诊断不同腹腔感染的潜力。