Kim Kyung Hoon, Hwang Ahreum, Song Younseong, Lee Wang Sik, Moon Jeong, Jeong Jinyoung, Bae Nam Ho, Jung Young Mee, Jung Jiyoung, Ryu Seunghwa, Lee Seok Jae, Choi Bong Gill, Kang Taejoon, Lee Kyoung G
Division of Nano-Bio Sensors/Chips Development, National NanoFab Center (NNFC), Daejeon 34141, Republic of Korea.
Bionanotechnology Research Center, Korea Research Institute of Bioscience & Biotechnology (KRIBB), Daejeon 34141, Republic of Korea.
ACS Nano. 2021 Mar 23;15(3):4777-4788. doi: 10.1021/acsnano.0c09411. Epub 2021 Jan 27.
Effective capture and rapid detection of pathogenic bacteria causing pandemic/epidemic diseases is an important task for global surveillance and prevention of human health threats. Here, we present an advanced approach for the on-site capture and detection of pathogenic bacteria through the combination of hierarchical nanostructures and a nuclease-responsive DNA probe. The specially designed hierarchical nanocilia and network structures on the pillar arrays, termed 3D bacterial capturing nanotopographical trap, exhibit excellent mechanical reliability and rapid (<30 s) and irreversible bacterial capturability. Moreover, the nuclease-responsive DNA probe enables the highly sensitive and extremely fast (<1 min) detection of bacteria. The bacterial capturing nanotopographical trap (b-CNT) facilitates the on-site capture and detection of notorious infectious pathogens ( O157:H7, , , and ) from kitchen tools and food samples. Accordingly, the usefulness of the b-CNT is confirmed as a simple, fast, sensitive, portable, and robust on-site capture and detection tool for point-of-care testing.
有效捕获和快速检测引发大流行/流行病的致病细菌是全球监测和预防人类健康威胁的一项重要任务。在此，我们展示了一种通过分层纳米结构与核酸酶响应性DNA探针相结合的先进方法，用于现场捕获和检测致病细菌。柱阵列上专门设计的分层纳米纤毛和网络结构，称为3D细菌捕获纳米拓扑陷阱，具有出色的机械可靠性以及快速（<30秒）且不可逆的细菌捕获能力。此外，核酸酶响应性DNA探针能够实现对细菌的高度灵敏且极其快速（<1分钟）的检测。细菌捕获纳米拓扑陷阱（b-CNT）有助于从厨房工具和食品样本中现场捕获和检测臭名昭著的传染性病原体（O157:H7等）。因此，b-CNT作为一种用于即时检测的简单、快速、灵敏、便携且强大的现场捕获和检测工具的实用性得到了证实。
