Quest Diagnostics Infectious Disease, San Juan Capistrano, CA, USA.
Quest Diagnostics Infectious Disease, San Juan Capistrano, CA, USA.
Diagn Microbiol Infect Dis. 2021 May;100(1):115303. doi: 10.1016/j.diagmicrobio.2020.115303. Epub 2020 Dec 29.
Published studies show that >99% of sera reactive in the reverse syphilis testing algorithm (RSTA) screening assay with an index above an assay-specific threshold confirm as reactive, with either a rapid plasma reagin-reactive (RPR) or RPR-nonreactive/Treponema pallidum particle agglutination-reactive (TPPA) result. However, the relationship between screen indices and confirmatory patterns has not been characterized. We thus assessed confirmatory testing results for 577 sera submitted for RSTA testing and a screen-reactive result in the DiaSorin Liaison assay. The median screen index was significantly higher for RPR samples than TPPA samples (55.6 versus 10.4), and the proportion with indices >28.3 (median for all 577 samples) was significantly higher for RPR versus TPPA samples (82% versus 26%). However, RPR titers did not significantly correlate with screen indices (R = 0.02). These findings demonstrate a significant relationship between RSTA screen indices and confirmatory assay results. The clinical utility of this relationship requires further study.
已发表的研究表明，在反向梅毒检测算法（RSTA）筛查检测中，超过 99%的血清学反应指数超过检测特异性阈值的样本，无论是快速血浆反应素阳性（RPR）还是 RPR 阴性/梅毒螺旋体颗粒凝集反应阳性（TPPA）的结果，都被确认为阳性。然而，目前尚未对 RSTA 筛查检测中血清学反应指数和确证模式之间的关系进行分析。因此，我们对 577 份血清样本进行了确证性检测，这些样本均在 DiaSorin Liaison 检测中呈现 RSTA 筛查阳性结果。RPR 样本的中位数筛查指数显著高于 TPPA 样本（55.6 比 10.4），RPR 样本中指数大于 28.3（所有 577 个样本的中位数）的比例显著高于 TPPA 样本（82%比 26%）。然而，RPR 滴度与筛查指数之间没有显著相关性（R=0.02）。这些发现表明 RSTA 筛查指数与确证检测结果之间存在显著关系。这种关系的临床应用价值需要进一步研究。
