Department of Obstetrics, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Nutrition and Foods Program, School of Family and Consumer Sciences, Texas State University, San Marcos, Texas, USA.
Ann Nutr Metab. 2020;76(6):431-440. doi: 10.1159/000507472. Epub 2021 Jan 27.
Choline-metabolizing genetic variation may interact with choline intake on fetal programming and pregnancy outcome. This case-control study aims to explore the association of maternal choline consumption and phosphatidylethanolamine N-methyltransferase (PEMT) gene polymorphism rs7946 with preterm birth risk.
145 Han Chinese women with preterm delivery and 157 Han Chinese women with term delivery were recruited in Shanghai. Dietary choline intake during pregnancy was assessed using a validated food frequency questionnaire. Additionally, DNA samples were genotyped for PEMT rs7946 (G5465A) with plasma homocysteine (Hcy) levels measured.
Compared with the lowest quartile of choline intake, women within the highest consumption quartile had adjusted odds ratio (aOR) for preterm birth of 0.48 (95% confidence interval, CI [0.24, 0.95]). There was a significant interaction between maternal choline intake and PEMT rs7946 (p for interaction = 0.04), where the AA genotype carriers who consumed the energy-adjusted choline <255.01 mg/day had aOR for preterm birth of 3.75 (95% CI [1.24, 11.35]), compared to those with GG genotype and choline intake >255.01 mg/day during pregnancy. Additionally, the greatest elevated plasma Hcy was found in the cases with AA genotype and choline consumption <255.01 mg/day (p < 0.001).
The AA genotype of PEMT rs7946 may be associated with increased preterm birth in these Han Chinese women with low choline intake during pregnancy.
胆碱代谢的遗传变异可能与胎儿发育和妊娠结局有关。本病例对照研究旨在探讨母体胆碱摄入与磷酸乙醇胺 N-甲基转移酶（PEMT）基因多态性 rs7946 与早产风险的关系。
在上海招募了 145 名早产汉族妇女和 157 名足月分娩汉族妇女。使用经过验证的食物频率问卷评估妊娠期间的膳食胆碱摄入量。此外，还对 PEMT rs7946（G5465A）进行了 DNA 基因分型，并测量了血浆同型半胱氨酸（Hcy）水平。
与胆碱摄入量最低的四分位数相比，摄入量最高的四分位数的妇女早产的调整优势比（aOR）为 0.48（95%置信区间 [0.24, 0.95]）。母体胆碱摄入量和 PEMT rs7946 之间存在显著的交互作用（p 交互=0.04），其中能量调整后的胆碱摄入量<255.01mg/天的 AA 基因型携带者早产的 aOR 为 3.75（95%CI [1.24, 11.35]），与 GG 基因型和怀孕期间胆碱摄入量>255.01mg/天的携带者相比。此外，在 AA 基因型且胆碱摄入量<255.01mg/天的病例中发现了最高的血浆 Hcy 升高（p<0.001）。
在这些妊娠期间胆碱摄入较低的汉族妇女中，PEMT rs7946 的 AA 基因型可能与早产风险增加有关。
