Cellular and Molecular Research Center, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran.
J Mol Neurosci. 2021 Jun;71(6):1301-1305. doi: 10.1007/s12031-020-01757-8. Epub 2021 Jan 27.
Twins studies indicate that many individual factors are associated with genetic polymorphisms in tobacco use, dependence vulnerability, and the ability to quit smoking. Opioid receptor delta-type 1 (OPRD1) is one of the most important genes in the opioid pathway. Therefore, the current study aimed to investigate the association of variants located in the intron 1 of the OPRD1 gene, including rs2236857, rs2236855, and rs760589, with susceptibility to nicotine dependence among northern Iranians. DNA of 426 individuals, including 224 smokers and 202 healthy people, were extracted with the salting-out standard technique, qualified with Agarose gel, then quantified with Nanodrop, and finally genotyped by Amplification Refractory Mutation System (ARMS) PCR. All statistical analyses were performed by SNPAlyze version 8.1 and SPSS version 20. Results revealed no significant association of all three studied variants with the susceptibility to nicotine dependence in any models of inheritance. However, there were five haplotypes with an overall frequency higher than 0.05; no significant impact of any of them on nicotine dependence was observed. Altogether, rs2236857, rs2236855, and rs760589 were not associated with nicotine dependence among northern Iranians.
双胞胎研究表明，许多个体因素与吸烟、依赖易感性和戒烟能力的遗传多态性有关。阿片受体 delta 型 1（OPRD1）是阿片途径中最重要的基因之一。因此，本研究旨在探讨位于 OPRD1 基因内含子 1 中的变异与伊朗北部人群尼古丁依赖易感性的关系，包括 rs2236857、rs2236855 和 rs760589。采用盐析标准技术提取 426 名个体（包括 224 名吸烟者和 202 名健康人）的 DNA，用琼脂糖凝胶进行质量检测，用 Nanodrop 进行定量，最后用扩增受阻突变系统（ARMS）PCR 进行基因分型。所有统计分析均采用 SNPAlyze 版本 8.1 和 SPSS 版本 20 进行。结果显示，在任何遗传模式下，所有三种研究变异均与尼古丁依赖易感性无显著关联。然而，有五个单倍型的总体频率高于 0.05；它们中的任何一个都没有对尼古丁依赖产生显著影响。总之，rs2236857、rs2236855 和 rs760589 与伊朗北部人群的尼古丁依赖无关。
