Department of Laboratory Medicine, University of California San Francisco, 1001 Potrero Ave, San Francisco, CA 94110, USA.
Department of Emergency Medicine, Baylor College of Medicine, 1 Baylor Plaza, Houston, TX 77030, USA.
J Anal Toxicol. 2022 Mar 21;46(3):303-321. doi: 10.1093/jat/bkab015.
Many natural products have biological effects on humans and animals. Poisoning caused by natural products is common in clinical toxicology cases. Liquid chromatography-high-resolution mass spectrometry (LC-HRMS) has recently emerged as a powerful analytical tool for large-scale target screening, and the application of LC-HRMS can be expanded to evaluate potential natural product poisoning in clinical cases. We report the construction of an LC-HRMS spectral library of 95 natural products commonly implicated in poisoning, and an LC-HRMS assay was validated for definitive detection of natural products in urine and serum samples. For each compound, the limit of detection was determined in the analytical range of 1.0-1,000 ng/mL for urine samples and 0.50-500 ng/mL for serum samples. The mean (SD) values of matrix effects for urine samples and that for serum samples were both -21% (22%), and the mean (SD) value of recovery for serum samples was 89% (26%). The LC-HRMS assay was successfully applied to identify natural products in clinical cases. The spectral library parameters of each compound are provided in the supplementary material to aid other laboratories in identification of unknown natural toxins and development of similar methods on different mass spectrometry platforms.
许多天然产物对人类和动物具有生物效应。在临床毒理学病例中，天然产物中毒很常见。液相色谱-高分辨率质谱（LC-HRMS）最近已成为大规模靶向筛选的强大分析工具，LC-HRMS 的应用可扩展到评估临床病例中潜在的天然产物中毒。我们报告了构建一个涉及 95 种常见中毒天然产物的 LC-HRMS 光谱库，并验证了 LC-HRMS 测定法可用于尿液和血清样本中天然产物的明确检测。对于每种化合物，在尿液样本的分析范围内（1.0-1,000ng/mL）和血清样本的分析范围内（0.50-500ng/mL）确定检测限。尿液样本和血清样本的基质效应平均值（SD）分别为-21%（22%），血清样本的平均（SD）回收率为 89%（26%）。LC-HRMS 测定法成功应用于鉴定临床病例中的天然产物。每个化合物的光谱库参数均在补充材料中提供，以帮助其他实验室鉴定未知的天然毒素，并在不同的质谱平台上开发类似的方法。
