Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Kitashirakawaoiwakecho, Sakyo-ku, Kyoto, Kyoto 606-8502, Japan.
Genuine R&D Company, Limited, 729-1 Matono, Shingu-machi, Kasuya-gun, Fukuoka 811-0104, Japan.
J Agric Food Chem. 2021 Aug 18;69(32):9188-9198. doi: 10.1021/acs.jafc.0c07259. Epub 2021 Jan 28.
Although the beneficial effects of dietary sphingolipids have recently been reported, the mechanism of their intestinal absorption has yet to be fully elucidated. In this study, the absorption and metabolism of dietary ceramides and glucosylceramides were evaluated by liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis in the plasma of mice after a single oral administration. Ceramide molecules prepared from soy sauce lees (mainly composed of phytosphingosine and its derivatives) were undetectable or minor compounds in the plasma of control mice but appeared 1-6 h after administration. Rice glucosylceramide (mainly composed of sphingadienine) was endogenously detected in mouse plasma and showed a tendency to increase 1-6 h after administration by LC-MS/MS analysis. In addition, the ceramide molecules, which are hydrolysates of dietary glucosylceramide, were significantly increased in the plasma after administration. These findings strongly suggest that dietary ceramides and glucosylceramides are partly absorbed as intact molecules or hydrolysates.
虽然膳食神经酰胺的有益作用最近已经被报道，但其肠道吸收的机制尚未完全阐明。在这项研究中，通过液相色谱-串联质谱（LC-MS/MS）分析，在小鼠单次口服后评估了膳食神经酰胺和神经鞘糖脂的吸收和代谢。从酱油渣（主要由植物鞘氨醇及其衍生物组成）中制备的神经酰胺分子在对照小鼠的血浆中是不可检测或次要的化合物，但在给药后 1-6 小时出现。米神经鞘糖脂（主要由神经鞘氨醇组成）在小鼠血浆中内源性检测到，通过 LC-MS/MS 分析显示给药后 1-6 小时有增加的趋势。此外，膳食神经鞘糖脂的水解产物神经酰胺分子在给药后血浆中的含量显著增加。这些发现强烈表明，膳食神经酰胺和神经鞘糖脂部分作为完整分子或水解产物被吸收。
