Center for University-Wide Education, School of Health and Social Services, Saitama Prefectural University, Koshigaya, Saitama, Japan.
Department of Health Sciences, School of Health and Social Services, Saitama Prefectural University, Koshigaya, Saitama, Japan.
PLoS One. 2021 Jan 28;16(1):e0243745. doi: 10.1371/journal.pone.0243745. eCollection 2021.
It has been reported that genetic factors are associated with risk factors and onset of lifestyle-related diseases, but this finding is still the subject of much debate.
The aim of the present study was to investigate the correlation of genetic factors, including salivary telomere length and three single nucleotide polymorphisms (SNPs) that may influence lifestyle-related diseases, with lifestyle-related diseases themselves.
In one year at a single facility, relative telomere length and SNPs were determined by using monochrome multiplex quantitative polymerase chain reaction and TaqMan SNP Genotyping Assays, respectively, and were compared with lifestyle-related diseases in 120 Japanese individuals near our university.
In men and all participants, age was inversely correlated with relative telomere length with respective p values of 0.049 and 0.034. In men, the frequency of hypertension was significantly higher in the short relative telomere length group than in the long group with unadjusted p value of 0.039, and the difference in the frequency of hypertension between the two groups was of borderline statistical significance after adjustment for age (p = 0.057). Furthermore, in men and all participants, the sum of the number of affected lifestyle-related diseases, including hypertension, was significantly higher in the short relative telomere length group than in the long group, with p values of 0.004 and 0.029, respectively. For ADIPOQ rs1501299, men's ankle brachial index was higher in the T/T genotype than in the G/G and G/T genotypes, with p values of 0.001 and 0.000, respectively. For SIRT1 rs7895833, men's body mass index and waist circumference and all participants' brachial-ankle pulse wave velocity were higher in the A/G genotype than in the G/G genotype, with respective p values of 0.048, 0.032 and 0.035. For FOXO3A rs2802292, women's body temperature and all participants' saturation of peripheral oxygen were lower in the G/T genotype than in the T/T genotype, with respective p values of 0.039 and 0.032. However, relative telomere length was not associated with physiological or anthropometric measurements except for height in men (p = 0.016). ADIPOQ rs1501299 in men, but not the other two SNPs, was significantly associated with the sum of the number of affected lifestyle-related diseases (p = 0.013), by genotype. For each SNPs, there was no significant difference in the frequency of hypertension or relative telomere length by genotype.
Relative telomere length and the three types of SNPs determined using saliva have been shown to be differentially associated with onset of and measured risk factors for lifestyle-related diseases consisting mainly of cardiovascular diseases and cancer.
有报道称，遗传因素与生活方式相关疾病的危险因素和发病有关，但这一发现仍存在争议。
本研究旨在探讨遗传因素（包括唾液端粒长度和三个可能影响生活方式相关疾病的单核苷酸多态性[SNP]）与生活方式相关疾病本身的相关性。
在一个设施中进行了一年的研究，使用单色多重定量聚合酶链反应和 TaqMan SNP 基因分型检测分别确定相对端粒长度和 SNP，并在我们大学附近的 120 名日本个体中与生活方式相关疾病进行了比较。
在男性和所有参与者中，年龄与相对端粒长度呈负相关，相应的 p 值分别为 0.049 和 0.034。在男性中，短相对端粒长度组的高血压发生率明显高于长相对端粒长度组，未校正 p 值为 0.039，校正年龄后两组高血压发生率差异有统计学意义（p = 0.057）。此外，在男性和所有参与者中，短相对端粒长度组的生活方式相关疾病（包括高血压）的总发病率明显高于长相对端粒长度组，p 值分别为 0.004 和 0.029。对于 ADIPOQ rs1501299，男性的踝臂指数在 T/T 基因型中高于 G/G 和 G/T 基因型，p 值分别为 0.001 和 0.000。对于 SIRT1 rs7895833，男性的体重指数和腰围以及所有参与者的肱踝脉搏波速度在 A/G 基因型中高于 G/G 基因型，相应的 p 值分别为 0.048、0.032 和 0.035。对于 FOXO3A rs2802292，女性的体温和所有参与者的外周血氧饱和度在 G/T 基因型中低于 T/T 基因型，相应的 p 值分别为 0.039 和 0.032。然而，除了男性的身高外，相对端粒长度与生理或人体测量学测量值无关（p = 0.016）。ADIPOQ rs1501299 仅在男性中，而不是其他两个 SNP，与生活方式相关疾病的发病和危险因素的总和显著相关（p = 0.013），这与基因型有关。对于每个 SNP，基因型之间高血压或相对端粒长度的频率没有显著差异。
唾液中确定的相对端粒长度和三种类型的 SNP 与主要由心血管疾病和癌症组成的生活方式相关疾病的发病和测量危险因素有差异相关。
