Maier R J, Prosser J
Department of Biology, Johns Hopkins University, Baltimore, Maryland 21218.
J Bacteriol. 1988 Apr;170(4):1986-9. doi: 10.1128/jb.170.4.1986-1989.1988.
Azotobacter vinelandii can grow mixotrophically with H2 plus mannose under N2-fixing conditions (T. Y. Wong and R. J. Maier, J. Bacteriol. 163:528-533, 1985). Mixotrophically grown cultures incubated in H2 transported mannose with a Vmax fourfold greater than that observed for cultures incubated in argon, but H2 did not change the apparent Km for mannose. Respiratory inhibitors, such as potassium cyanide, hydroxylamine, and p-chloromercuribenzoic acid, as well as the proton conductor carbonyl cyanide m-chlorophenyl-hydrazone inhibited mannose uptake. We suggest that one of the roles of H2 in mixotrophic metabolism is to supply energy that facilitates mannose transport.
棕色固氮菌在固氮条件下可以利用氢气和甘露糖进行混合营养生长（T. Y. 黄和R. J. 迈尔，《细菌学杂志》163:528 - 533, 1985）。在氢气中培养的混合营养生长培养物运输甘露糖的最大反应速度比在氩气中培养的培养物高四倍，但氢气并没有改变甘露糖的表观米氏常数。呼吸抑制剂，如氰化钾、羟胺和对氯汞苯甲酸，以及质子导体羰基氰化物间氯苯腙抑制了甘露糖的摄取。我们认为氢气在混合营养代谢中的作用之一是提供能量以促进甘露糖的运输。
