Department of Chemistry, Adam Mickiewicz University, Uniwersytetu Poznańskiego 8, 61-614 Poznań, Poland.
Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704 Poznań, Poland.
Org Biomol Chem. 2021 Feb 18;19(6):1292-1295. doi: 10.1039/d0ob02422h.
We describe highly efficient interstrand photocrosslinking of a DNA duplex containing 5-chloro-2'-deoxy-4-thiouridine (ClSdU) in one strand, proceeding via a two-step photochemical cascade, involving the formation of a thermally reversible crosslink between ClSdU and thymidine in the target strand and its subsequent conversion to a thermally stable fluorescent crosslink. These results show that ClSdU has great potential to be a valuable DNA photo-crosslinking reagent for chemical biology applications.
我们描述了一种含有 5-氯-2'-脱氧-4-硫尿苷（ClSdU）的 DNA 双链体的高效链间光交联，该过程通过两步光化学级联进行，涉及 ClSdU 与靶链中胸腺嘧啶之间形成热可逆交联及其随后转化为热稳定的荧光交联。这些结果表明，ClSdU 很有潜力成为用于化学生物学应用的有价值的 DNA 光交联试剂。
