Sumar Alice H S, Vieira Ariany M, Martins Gisele H C, Probst Jéssica J, Martins Daniel F, Bobinski Franciane, Horewicz Verônica V, Mack Josiel M, Danielli Naissa M, Cunha Mauricio P, Assreuy Jamil, Bonorino Kelly C, Goethel Márcio F, Domenech Susana C, Dafre Alcir L, Kunzler Deborah de C H
Department of Physical Therapy, Laboratory of Experimental Research and Graduate Program in Physiotherapy (PPGFt/CEFID), Universidade do Estado de Santa Catarina - UDESC, Florianópolis, SC, Brazil.
Graduate Program in Health Sciences, Laboratory of Experimental Neuroscience (LaNEx), Universidade do Sul de Santa Catarina - UNISUL, Palhoça, SC, Brazil.
Cytokine. 2021 Apr;140:155401. doi: 10.1016/j.cyto.2020.155401. Epub 2021 Jan 25.
Sepsis is a potentially deadly organic dysfunction, and one of the main causes of mortality in intensive care units (ICU). Aerobic exercise (AE) is a preventive intervention in the establishment of inflammatory conditions, such as chronic lung diseases, but its effects on sepsis remain unclear. Therefore, this study aimed to evaluate the effects of AE on health condition, mortality, inflammation, and oxidative damage in an experimental model of pneumosepsis induced by Klebsiella pneumoniae (K.p).
Animals were randomly allocated to Control; Exercise (EXE); Pneumosepsis (PS) or Exercise + Pneumosepsis (EPS) groups. Exercised animals were submitted to treadmill exercise for 2 weeks, 30 min/day, prior to pneumosepsis induced by K.p tracheal instillation.
PS produced a striking decrease in the health condition leading to massive death (85%). AE protected mice, as evidenced by better clinical scores and increased survival (70%). AE alleviated sickness behavior in EPS mice as evaluated in the open field test, and inflammation (nitrite + nitrate, TNF-α and IL-1β levels) in broncoalveolar fluid. Catalase activity, oxidative damage to proteins and DNA was increased by sepsis and prevented by exercise.
Overall, the beneficial effects of exercise in septic animals encompassed a markedly improved clinical score and decreased mortality, along with lower inflammation markers, less DNA and protein damage, as well as preserved antioxidant enzyme activity. Neural network risk analysis revealed exercise had a considerable effect on the overall health condition of septic mice.
脓毒症是一种潜在致命的器官功能障碍，是重症监护病房（ICU）患者死亡的主要原因之一。有氧运动（AE）是一种针对慢性肺部疾病等炎症性疾病的预防性干预措施，但其对脓毒症的影响尚不清楚。因此，本研究旨在评估有氧运动对肺炎克雷伯菌（K.p）诱导的肺炎脓毒症实验模型中健康状况、死亡率、炎症和氧化损伤的影响。
将动物随机分为对照组、运动组（EXE）、肺炎脓毒症组（PS）或运动+肺炎脓毒症组（EPS）。在通过气管内滴注K.p诱导肺炎脓毒症之前，对运动组动物进行为期2周、每天30分钟的跑步机运动。
肺炎脓毒症导致健康状况显著下降，引发大量死亡（85%）。有氧运动对小鼠起到了保护作用，表现为更好的临床评分和更高的存活率（70%）。在旷场试验中评估发现，有氧运动减轻了运动+肺炎脓毒症组小鼠的疾病行为，以及支气管肺泡灌洗液中的炎症（亚硝酸盐+硝酸盐、TNF-α和IL-1β水平）。脓毒症会增加过氧化氢酶活性、蛋白质和DNA的氧化损伤，而运动可预防这些损伤。
总体而言，运动对脓毒症动物的有益影响包括临床评分显著改善、死亡率降低、炎症标志物水平降低、DNA和蛋白质损伤减少以及抗氧化酶活性保持。神经网络风险分析显示，运动对脓毒症小鼠的整体健康状况有显著影响。
