School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough, Leicestershire LE12 5RD, UK.
School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough, Leicestershire LE12 5RD, UK.
Food Res Int. 2021 Jan;139:109948. doi: 10.1016/j.foodres.2020.109948. Epub 2020 Dec 8.
The establishment of the INFOGEST in vitro static digestion method, a standardized international consensus, was an important milestone in the field of food digestion. We evaluated the contribution of iron and zinc in reagents used in the INFOGEST method in relation to sample iron and zinc and the potential interference of reagent-derived iron and zinc with bioaccessibility measurements. In most cases, reagent-derived iron and zinc contributed more than 50% of the total iron or zinc in the digesta containing selected cereals and legumes. Moreover, the chemical behaviour of reagent-derived iron and zinc was matrix dependent such that the application of a blanket blank correction was not appropriate. We therefore propose an improved approach involving isotopic labelling of reagent iron and zinc in order to discriminate between reagent-derived and sample-derived iron and zinc in each matrix. This stable isotope approach could improve the accuracy and reliability of iron and zinc bioaccessibility studies.
INFOGEST 体外静态消化法的建立是食品消化领域的一个重要里程碑，该方法是标准化的国际共识。我们评估了 INFOGEST 方法中试剂中铁和锌的含量对样品中铁和锌的贡献，以及试剂中铁和锌对生物可利用性测量的潜在干扰。在大多数情况下，与包含选定谷物和豆类的消化物中的总铁或总锌相比，试剂中衍生的铁和锌的贡献超过 50%。此外，试剂中衍生的铁和锌的化学行为取决于基质，因此不适合应用通用空白校正。因此，我们提出了一种改进的方法，即用同位素标记试剂中的铁和锌，以便在每个基质中区分试剂衍生和样品衍生的铁和锌。这种稳定同位素方法可以提高铁和锌生物利用度研究的准确性和可靠性。
