Kosgey Zennah C, Edae Erena A, Dill-Macky Ruth, Jin Yue, Bulbula Worku Denbel, Gemechu Ashenafi, Macharia Godwin, Bhavani Sridhar, Randhawa Mandeep S, Rouse Matthew N
Kenya Agricultural and Livestock Research Organization, Njoro, Kenya.
Department of Plant Pathology, University of Minnesota, Saint Paul, MN, United States.
Front Plant Sci. 2021 Jan 12;11:609659. doi: 10.3389/fpls.2020.609659. eCollection 2020.
Stem rust caused by f. sp. () remains a constraint to wheat production in East Africa. In this study, we characterized the genetics of stem rust resistance, identified QTLs, and described markers associated with stem rust resistance in the spring wheat line CI 14275. The 113 recombinant inbred lines, together with their parents, were evaluated at the seedling stage against races TTKSK, TRTTF, TPMKC, TTTTF, and RTQQC. Screening for resistance to races in the field was undertaken in Kenya, Ethiopia, and the United States in 2016, 2017, and 2018. One gene conferred seedling resistance to race TTTTF, likely . Three QTL were identified that conferred field resistance. QTL , that conferred resistance in Kenya and Ethiopia, was validated, and the marker Excalibur_c7963_1722 was shown to have potential to select for this QTL in marker-assisted selection. The QTL is likely , and appears to be a new QTL. This is the first study to both detect and validate an adult plant stem rust resistance QTL on chromosome arm 2BS. The combination of field QTL , , and has the potential to be used in wheat breeding to improve stem rust resistance of wheat varieties.
由禾柄锈菌小麦专化型（Puccinia graminis f. sp. tritici）引起的秆锈病仍然是东非小麦生产的一个制约因素。在本研究中，我们对春小麦品系CI 14275中秆锈病抗性的遗传特性进行了表征，鉴定了数量性状基因座（QTL），并描述了与秆锈病抗性相关的分子标记。113个重组自交系及其亲本在苗期针对TTKSK、TRTTF、TPMKC、TTTTF和RTQQC菌系进行了评估。2016年、2017年和2018年在肯尼亚、埃塞俄比亚和美国进行了田间对秆锈菌系抗性的筛选。一个基因赋予了对TTTTF菌系的苗期抗性，可能是Sr36。鉴定出了3个赋予田间抗性的QTL。在肯尼亚和埃塞俄比亚赋予抗性的QTL QSr.cim-2BS，得到了验证，并且分子标记Excalibur_c7963_1722在标记辅助选择中显示出有潜力用于选择该QTL。QTL QSr.cim-2DS可能是Sr2，而QSr.cim-6DS似乎是一个新的QTL。这是第一项同时检测和验证2BS染色体臂上成年植株秆锈病抗性QTL的研究。田间QTL QSr.cim-2BS、QSr.cim-2DS和QSr.cim-6DS的组合有潜力用于小麦育种，以提高小麦品种的秆锈病抗性。
