Programa de Pós-Graduação em Biologia Experimental, Universidade Federal de Rondônia, Porto Velho, RO, Brazil.
Laboratório de Bioecologia de Insetos, Universidade Federal de Rondônia, Porto Velho, RO, Brazil.
J Med Entomol. 2021 May 15;58(3):1264-1269. doi: 10.1093/jme/tjaa265.
In the search for new strategies to control Aedes aegypti Linnaeus (Diptera: Culicidae), several studies have successfully related pyriproxyfen (PPF) tarsal transference to breeding sites (autodissemination), as well as the sterilization potential of females exposed to PPF. Potential PPF autodissemination by mosquito feces after the ingestion of sugar baits has also been proposed. Therefore, the present work evaluated several parameters, e.g., fecal production, residuality under dry and aqueous conditions, PPF excretion affecting emergence inhibition (EI) by fecal deposits of Ae. aegypti fed with attractive toxic sugar baits (ATSBs) containing PPF as well as their reproductive potential. Females were fed with ATSBs offered as droplets and the feces were collected using filter paper and transferred to plastic cups with L3 larvae to evaluate EI. The residual effect of feces in aqueous and dry conditions and PPF excretion on EI was obtained by keeping the feces in water or dried for different time intervals and using feces collected at 24-h intervals, respectively. Females received a bloodmeal after feeding on ATSBs, eggs and larval counting expressed the reproductive potential. The fecal mass was not affected by PPF concentration, but EI increased from 33 to 54% as the PPF concentration increased. The PPF excretion in the feces exceeded 96 h. The residual effect in the EI for feces kept in water was reduced by more than 60% after 30 d but was not affected under dry conditions. The fecundity and fertility of the females were reduced up to 51% and 97%, respectively.
在寻找控制埃及伊蚊（双翅目：库蚊科）的新策略时，已有多项研究成功将吡丙醚（PPF）跗节转移与繁殖地（自传播）相关联，以及接触 PPF 的雌性的绝育潜力。还提出了蚊子在摄入糖诱饵后通过粪便排出 PPF 的潜在自传播能力。因此，本研究评估了几个参数，例如粪便产生量、在干燥和水条件下的残留性、摄入含有 PPF 的有吸引力的毒性糖诱饵（ATSB）的埃及伊蚊雌蚊的 PPF 排泄对粪便沉积引起的出蚊抑制（EI）的影响以及它们的生殖潜力。雌性以液滴形式喂食 ATSB，并用滤纸收集粪便，并将其转移到装有 L3 幼虫的塑料杯中，以评估 EI。通过将粪便保存在水中或在不同的时间间隔内干燥，以及分别使用在 24 小时间隔收集的粪便来获得在水和干燥条件下的粪便残留效果以及 PPF 排泄对 EI 的影响。在喂食 ATSB 后，雌性接受了一次血餐，产卵和幼虫计数表示生殖潜力。粪便质量不受 PPF 浓度的影响，但随着 PPF 浓度的增加，EI 从 33%增加到 54%。粪便中 PPF 的排泄超过 96 小时。在水中保存的粪便的残留效果在 30 天后降低了 60%以上，但在干燥条件下不受影响。雌性的繁殖力和生育力分别降低了 51%和 97%。
