Research Division for Biotechnology, Advanced Radiation Technology Institute (ARTI), Korea Atomic Energy Research Institute (KAERI), Jeongeup-si, Jeollabuk-do, 56212, Republic of Korea.
Radiation Biotechnology and Applied Radioisotope Science, University of Science and Technology (UST), Daejeon, 34113, South Korea.
Mol Biol Rep. 2021 Feb;48(2):1081-1091. doi: 10.1007/s11033-021-06156-9. Epub 2021 Jan 28.
Melanoma is aggressive, highly metastatic, and potentially fatal. In the case of patients with advanced melanoma, it is difficult to expect a good prognosis, since this cancer has low sensitivity to chemotherapy and radiation therapy. The use of natural ingredients may enhance existing therapies. Centipedegrass extract (CGE) which contains phenolic structures and C-glycosyl flavones, has been shown to have anti-inflammatory effects and anti-cancer effects. The purpose of this study was to evaluate the radio sensitizing effects of CGE in combination with ionizing radiation (IR). Two melanoma cell lines were exposed to IR after treatment with CGE at concentrations that were not toxic alone. The effects of CGE + IR on cell survival, cell cycle, and apoptotic cell death were examined using MTT and Muse® Cell Analyzer, and fluorescence microscopy. Molecular signaling mechanisms were explored by western blots. Our findings showed that co-treatment of CGE + IR reduced the survival of melanoma cells more than IR alone. Also, cell cycle arrest in CGE-treated cells was enhanced and these cells became more radiosensitive. CGE + IR increased apoptotic cell death more than IR alone. Western blot results showed that the effect of CGE + IR involved MAPKs (ERK1/2, p38, and JNK) pathway. Our study suggests that CGE + IR treatment enhanced radio-sensitization and cell death of melanoma cells via cell cycle arrest and the MAPKs pathway.
黑色素瘤具有侵袭性、高度转移性，并且可能致命。对于晚期黑色素瘤患者，预后往往不容乐观，因为这种癌症对化疗和放疗的敏感性较低。使用天然成分可能会增强现有疗法的效果。蜈蚣草提取物（CGE）含有酚类结构和 C-糖苷黄酮，已被证明具有抗炎和抗癌作用。本研究旨在评估 CGE 与电离辐射（IR）联合使用的放射增敏作用。在将 CGE 以单独使用时无毒的浓度处理两种黑色素瘤细胞系后，用 IR 暴露它们。使用 MTT 和 Muse®细胞分析仪以及荧光显微镜检查 CGE+IR 对细胞存活、细胞周期和凋亡细胞死亡的影响。通过 Western blot 探索分子信号转导机制。我们的研究结果表明，CGE+IR 联合治疗比单独使用 IR 更能降低黑色素瘤细胞的存活率。此外，CGE 处理的细胞中的细胞周期停滞得到增强，这些细胞对辐射更敏感。CGE+IR 诱导的细胞凋亡死亡比单独使用 IR 更多。Western blot 结果表明，CGE+IR 的作用涉及 MAPKs（ERK1/2、p38 和 JNK）通路。我们的研究表明，CGE+IR 治疗通过细胞周期停滞和 MAPKs 通路增强了黑色素瘤细胞的放射增敏作用和细胞死亡。
