Key Laboratory of Zoonoses Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, P. R. China.
College of Animal Science, Yangtze University, Jingzhou 434023, P. R. China.
ACS Appl Mater Interfaces. 2021 Feb 10;13(5):6091-6098. doi: 10.1021/acsami.0c21760. Epub 2021 Jan 29.
Convenient and ultrasensitive detection of pesticides is demanded for healthcare and environmental monitoring, which can be realized with a dual-modal strategy. In this paper, based on a biotin-labeled IgG-modified gold nanoparticle (AuNP@IgG-bio) probe, a dual-modal immunosensor was proposed for detecting chloroacetamide herbicides. This platform is relied on the dephosphorylation of ascorbic acid 2-phosphate (AA2P) by alkaline phosphatase (ALP). In addition to this process, ascorbic acid (AA)-triggered deposition of silver on gold nanostars (AuNSs) and the fluorogenic reaction of dehydrogenated AA and -phenylenediamine (OPD) occur sequentially. Thus, the dual readout of the color change of red-green-blue (RGB) and fluorescence generation in situ induced by crystal growth can be used. The limits of detection (LODs) were as low as 1.20 ng/mL of acetochlor (ATC), 0.89 ng/mL of metolachlor, 1.22 ng/mL of propisochlor, and 0.99 ng/mL of their mixture by a smartphone and 0.44 ng/mL of ATC, 1.59 ng/mL of metolachlor, 2.80 ng/mL of propisochlor, and 0.72 ng/mL of their mixture by a spectrofluorometer. The recoveries from corn were 91.4-105.1% of the colorimetric mode and 92.4-106.2% of the fluorescent mode. Due to its simple observation mode and good performance, this dual-modal immunosensor possesses considerable application prospects.
方便、灵敏地检测农药对于医疗保健和环境监测至关重要，而双模态策略可以实现这一目标。在本文中，基于生物素标记的 IgG 修饰金纳米粒子（AuNP@IgG-bio）探针，构建了一种用于检测氯乙酰胺类除草剂的双模态免疫传感器。该平台依赖于碱性磷酸酶（ALP）对抗坏血酸 2-磷酸（AA2P）的去磷酸化。除了这个过程，抗坏血酸（AA）引发金纳米星（AuNSs）上的银沉积，以及脱氢 AA 和 -苯二胺（OPD）的荧光发生反应，依次进行。因此，可以进行颜色变化的双读取（红-绿-蓝（RGB））和原位荧光生成，这是由晶体生长诱导的。通过智能手机，检测限（LOD）低至 1.20ng/mL 的乙草胺（ATC）、0.89ng/mL 的异丙甲草胺、1.22ng/mL 的异丙草胺和 0.99ng/mL 的混合物，以及 0.44ng/mL 的 ATC、1.59ng/mL 的异丙甲草胺、2.80ng/mL 的异丙草胺和 0.72ng/mL 的混合物。通过分光光度计，从玉米中回收的比色模式回收率为 91.4-105.1%，荧光模式回收率为 92.4-106.2%。由于其简单的观察模式和良好的性能，这种双模态免疫传感器具有相当大的应用前景。
