Co-application of Se and a biostimulant at different wheat growth stages: Influence on grain development.

An appropriate selenium intake can be beneficial for human health. Se-biofortified food in Se-deficient regions is becoming an increasingly common practice but there are still issues to be addressed regarding the observed Se-induced toxicity to the plant. In this respect, plant biostimulants are used to enhance nutrition efficiency, abiotic stress tolerance and crop quality. In this work, the efficacy of a plant biostimulant to counteract the Se-induced stress in wheat plants is experimentally assessed. The co-application of different Se-biofortification treatments and the biostimulant at different growth stages (tillering or heading stage) was investigated. The use of micro focused X-ray spectroscopy allows us to confirm organic Se species to be the main Se species found in wheat grain and that the proportion of organic Se species is only slightly affected by the Se application stage. Our study proves that the biostimulant had a key role in the enhancement of both the amount of grains produced per spike and their dry biomass without hindering Se enrichment process, neither diminishing the Se concentration nor massively disrupting the Se species present. This information will be useful to minimize both plant toxicity and economic cost towards a more effective and plant healthy selenium supplementation.