Robl J M, Dziuk P J
University of Illinois, Department of Animal Sciences, Urbana 61801.
J Exp Zool. 1988 Jan;245(1):97-101. doi: 10.1002/jez.1402450114.
This study attempted to determine a basis for the previously observed greater sensitivity of heterospermic tests when compared to homospermic tests for detecting differences in fertility between males. In theory, the results of heterospermic tests are an indication of the proportion of eggs fertilized per unit time whereas results of homospermic inseminations measure only the cumulative or final proportion of eggs fertilized. The fertilizing ability of sperm from males of CF1 and C57BL/6N strains of mice was compared homospermically using both relatively high and low concentrations of sperm and by measuring the proportion of eggs penetrated per unit of time. The fertilizing ability of sperm from these strains was also compared using heterospermic inseminations. When females were inseminated with a high concentration of sperm, males of both strains fertilized a high and indistinguishable percentage of eggs when examined after 30 hr. When females were inseminated with either a low concentration of sperm or when the proportion of eggs penetrated was measured at 5 hr, differences between strains of mice were distinguishable. Heterospermic insemination further magnified the observed difference between strains. The results of this study confirm that measuring the percentage of eggs fertilized per unit of time can enhance the magnitude of differences between males in fertility as compared to measuring only the final percentage of eggs fertilized. Measuring the percentage of eggs fertilized per unit of time does not, however, entirely account for the large differences observed between fertility of males when they are compared using heterospermic inseminations.
本研究试图确定一个依据，以解释为何先前观察到在检测雄性生育力差异时，异精受孕试验比同精受孕试验具有更高的敏感性。理论上，异精受孕试验的结果表明单位时间内受精的卵子比例，而同精受孕的结果仅测量受精卵子的累积或最终比例。使用相对高浓度和低浓度的精子，通过测量单位时间内穿透卵子的比例，对CF1和C57BL/6N品系小鼠雄性的精子受精能力进行了同精受孕比较。还使用异精受孕比较了这些品系精子的受精能力。当用高浓度精子对雌性进行授精时，在30小时后检查时，两个品系的雄性使卵子受精的比例都很高且无明显差异。当用低浓度精子对雌性进行授精时，或者在5小时测量穿透卵子的比例时，小鼠品系之间的差异是可区分的。异精受孕进一步放大了品系间观察到的差异。本研究结果证实，与仅测量最终受精卵子百分比相比，测量单位时间内受精卵子的百分比可以增强雄性生育力差异的幅度。然而，测量单位时间内受精卵子的百分比并不能完全解释在使用异精受孕比较雄性生育力时观察到的巨大差异。
