Department of Pediatrics, Xiangya Hospital, Central South University, Changsha, 410008, Hunan, People's Republic of China.
Newborn Department of Pediatrics, Renmin Hospital of Wuhan University, Wuhan, 430000, Hubei, People's Republic of China.
Sci Rep. 2021 Jan 29;11(1):2668. doi: 10.1038/s41598-021-82096-5.
Our aim was to detect type 2 innate lymphoid cells (ILC2s)-related cytokines of infants with bronchiolitis by using Elisa, Liquidchip technology and RT-PCR and investigated its correlation with bronchiolitis. We recruited 26 infants with bronchiolitis and 20 healthy infants as control from Xiangya Hospital. Compared to the control group, the serum levels of interleukin-5 (IL-5) [41.99 (21.11) vs 25.70 (19.64)], IL-9 [27.04 (37.51) vs 8.30 (0.54)], IL-13 [184.05 (132.81) vs 121.75 (176.13)], IL-33 [83.70 (46.69) vs 11.23 (55.31)] and thymic stromal lymphopoietin (TSLP) [31.42 (5.41) vs 28.76 (2.56)] were significantly increased in infants with bronchiolitis (P < 0.05), while the level of IgE had no significant difference between the two groups [19.05 (14.15) vs 14.85 (20.2), P > 0.05]. The mRNA expression of IL-17RB (9.83 ± 0.35 vs 9.19 ± 0.58), TSLP (16.98 ± 2.12 vs 15.07 ± 2.25), retinoid acid receptor related orphan receptor α (7.18 ± 0.71 vs 5.46 ± 1.09) and trans-acting T-cell-specific transcription factor 3 (4.86 ± 0.66 vs 4.19 ± 0.90) were significantly increased in infants with bronchiolitis versus the control group (P < 0.05), while there was no statistical significance for suppression of tumorigenicity 2 (5.59 ± 0.68 vs 5.41 ± 0.87, P > 0.05). Our findings suggested that ILC2s possibly play a specific role in immunopathology of bronchiolitis.
我们的目的是通过酶联免疫吸附试验（ELISA）、液芯芯片技术和 RT-PCR 检测毛细支气管炎患儿 2 型固有淋巴细胞（ILC2）相关细胞因子，并探讨其与毛细支气管炎的相关性。我们招募了 26 名毛细支气管炎患儿和 20 名健康婴儿作为对照，来自湘雅医院。与对照组相比，支气管肺炎患儿血清中白细胞介素-5（IL-5）[41.99（21.11）vs 25.70（19.64）]、白细胞介素-9（IL-9）[27.04（37.51）vs 8.30（0.54）]、白细胞介素-13（IL-13）[184.05（132.81）vs 121.75（176.13）]、白细胞介素-33（IL-33）[83.70（46.69）vs 11.23（55.31）]和胸腺基质淋巴生成素（TSLP）[31.42（5.41）vs 28.76（2.56）]明显升高（P < 0.05），而两组间 IgE 水平无明显差异[19.05（14.15）vs 14.85（20.2），P > 0.05]。IL-17RB（9.83 ± 0.35 vs 9.19 ± 0.58）、TSLP（16.98 ± 2.12 vs 15.07 ± 2.25）、视黄酸受体相关孤儿受体α（7.18 ± 0.71 vs 5.46 ± 1.09）和转录激活 T 细胞特异性转录因子 3（4.86 ± 0.66 vs 4.19 ± 0.90）的 mRNA 表达在支气管肺炎患儿中明显升高（P < 0.05），而肿瘤抑制因子 2 的表达无统计学意义（5.59 ± 0.68 vs 5.41 ± 0.87，P > 0.05）。我们的研究结果表明，ILC2 可能在毛细支气管炎的免疫病理学中发挥特定作用。
