State Key Laboratory of Natural Medicines, Jiangsu Key Laboratory of Bioactive Natural Product Research, School of Traditional Chinese Pharmacy, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing 210009, PR China.
State Key Laboratory of Natural Medicines, Jiangsu Key Laboratory of Bioactive Natural Product Research, School of Traditional Chinese Pharmacy, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing 210009, PR China.
Fitoterapia. 2021 Apr;150:104837. doi: 10.1016/j.fitote.2021.104837. Epub 2021 Jan 27.
Two new phthalide dimers (1 and 2) were obtained from the rhizomes of Ligusticum sinense Oliv., along with three known dimeric phthalides (3-5). Their structures were determined with the aid of the spectroscopic data, and their absolute configurations were elucidated based on the comparison of calculated and experimental electronic circular dichroism (ECD) spectra. All the compounds were evaluated in vitro for their inhibitory activities against NO production in LPS-treated RAW264.7 macrophages. Among them, compounds 1 and 3 showed potent NO prohibitive activity with IC values at 4.86 ± 0.29 μM and 4.87 ± 0.32 μM, respectively.
从当归的根茎中分离得到了两个新的苯酞二聚体（1 和 2），以及三个已知的苯酞二聚体（3-5）。通过光谱数据确定了它们的结构，并根据计算和实验电子圆二色性（ECD）光谱的比较阐明了它们的绝对构型。所有化合物均在体外评估了其对 LPS 处理的 RAW264.7 巨噬细胞中 NO 产生的抑制活性。其中，化合物 1 和 3 表现出较强的 NO 抑制活性，IC 值分别为 4.86±0.29 μM 和 4.87±0.32 μM。
