Department of Animal and Marine Bioresource Sciences, Faculty of Agriculture, Kyushu University, 744 Moto-oka, Nishi-ku, Fukuoka, 819-0395, Japan.
Department of Animal and Marine Bioresource Sciences, Faculty of Agriculture, Kyushu University, 744 Moto-oka, Nishi-ku, Fukuoka, 819-0395, Japan; Department of Animal Nutrition, Genetics and Breeding, Faculty of Animal Science and Veterinary Medicine, Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagor, Dhaka, 1207, Bangladesh.
Anim Reprod Sci. 2021 Mar;226:106696. doi: 10.1016/j.anireprosci.2021.106696. Epub 2021 Jan 15.
Interferon tau (IFNT) is thought to have essential functions in maternal recognition and establishment of pregnancy in ruminants. There, however, is a lack of research on embryonic factors that affect pregnancy other than IFNT. The present study was conducted to determine what are other embryo-derived factors involved in pregnancy recognition and to identify effects on endometrial cells using an in vitro culture system. With use of LC-MS/MS procedures to evaluate the supernatant of elongating embryos of cattle in culture, there was detection of 78 secretary proteins including five cytokines and two growth factors. Then there was analysis for up-regulated genes using ingenuity pathway procedure, IFNT and MIF were identified as upstream regulators of 37 and five genes, respectively. The mRNA transcript of MIF receptors was identified in endometrial cells, however, not in embryos. Among genes induced by MIF, CCL2, IL7 and IL23A transcripts were identified in endometrial cells. When endometrial cells were treated with interferon alpha (IFNA) and MIF, the CCL2 transcript was in a larger abundance of endometrial epithelial and polymorphonuclear neutrophil cells, and there was a larger abundance of there mRNA transcripts as a result of MIF treatment of peripheral blood mononuclear cells. In conclusion, MIF secreted by elongating embryos of cattle synergistically regulates relative abundances of specific mRNA transcripts of endometrial cells when there is treatment with IFNA, indicating further there are several factors other than IFNT that have effects on gene expression in the endometrium during early stages of gestation in cattle.
干扰素 tau（IFNT）被认为在反刍动物的母体识别和妊娠建立中具有重要功能。然而，除了 IFNT 之外，关于影响妊娠的胚胎因素的研究还很少。本研究旨在确定除 IFNT 之外，还有哪些胚胎衍生因素参与妊娠识别，并使用体外培养系统来确定对子宫内膜细胞的影响。使用 LC-MS/MS 程序评估培养中的牛伸长胚胎的上清液，检测到包括五种细胞因子和两种生长因子在内的 78 种分泌蛋白。然后使用 ingenuity pathway 程序分析上调基因，发现 IFNT 和 MIF 分别是 37 个和 5 个基因的上游调节剂。在子宫内膜细胞中鉴定到 MIF 受体的 mRNA 转录本，但在胚胎中没有。在 MIF 诱导的基因中，鉴定到 CCL2、IL7 和 IL23A 的转录本在子宫内膜细胞中。当子宫内膜细胞用干扰素 alpha（IFNA）和 MIF 处理时，CCL2 转录本在子宫内膜上皮细胞和多形核中性粒细胞中的丰度更大，并且由于 MIF 处理外周血单核细胞，存在更多的 mRNA 转录本。总之，牛伸长胚胎分泌的 MIF 与 IFNA 处理协同调节子宫内膜细胞特定 mRNA 转录本的相对丰度，表明除 IFNT 之外，还有几种因素对牛妊娠早期子宫内膜中的基因表达有影响。
