Minarrieta Lucía, Velasquez Lis Noelia, Sparwasser Tim, Berod Luciana
Ottawa Institute of Systems Biology, Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine, University of Ottawa, Ottawa, ON, K1H 8M5, Canada.
Institute of Medical Microbiology and Hygiene, University Medical Centre of the Johannes Gutenberg-University Mainz, Mainz, Germany.
Curr Opin Biotechnol. 2021 Apr;68:202-212. doi: 10.1016/j.copbio.2020.12.010. Epub 2021 Jan 28.
Dendritic cells (DCs) are key orchestrators of immunity and tolerance. It has become evident that DC function can be influenced by cellular metabolic programs. However, conclusions from early metabolic studies using in vitro GM-CSF DC cultures fail to correlate with bona fide DC populations. Here, we discuss the existing paradigms in the DC metabolism field, focusing on the limitations of the models utilized. Furthermore, we introduce alternative models to generate DCs in vitro that better emulate DCs found in vivo. Finally, we highlight new techniques to evaluate DC metabolism at the single-cell level. The combination of these two strategies could help advance the DC metabolism field towards a more physiological understanding, which is crucial for the development of effective DC-based therapies.
树突状细胞（DCs）是免疫和耐受的关键协调者。越来越明显的是，DC功能可受细胞代谢程序的影响。然而，早期使用体外GM-CSF DC培养物进行的代谢研究得出的结论与真正的DC群体并不相关。在此，我们讨论DC代谢领域的现有范式，重点关注所使用模型的局限性。此外，我们介绍了在体外生成DC的替代模型，这些模型能更好地模拟体内发现的DC。最后，我们强调了在单细胞水平评估DC代谢的新技术。这两种策略的结合有助于推动DC代谢领域朝着更符合生理的理解方向发展，这对于开发有效的基于DC的疗法至关重要。
