Laboratory of Physical Properties, Department of Chemical and Food Engineering, Federal University of Santa Catarina, Florianópolis, SC, 88040-970, Brazil.
Laboratory of Membrane Processes, Department of Chemical and Food Engineering, Federal University of Santa Catarina, Florianópolis, SC, 88040-970, Brazil.
Plant Foods Hum Nutr. 2021 Mar;76(1):90-97. doi: 10.1007/s11130-021-00878-8. Epub 2021 Jan 30.
Jambolan is an unexplored fruit rich in bioactive compounds like anthocyanins, catechin, and gallic acid. Thus, the extraction of bioactive compounds allows adding value to the fruit. In this context, the present study reports the recovery and concentration of jambolan fruit extract by ultra and nanofiltration for the first time. Acidified water was used to extract polyphenols from the pulp and peel of jambolan. The extracts were concentrated using ultrafiltration and nanofiltration membranes with nominal molecular weight cut-off ranging from 180 to 4000 g mol. Total monomeric anthocyanin, total phenolic compounds, and antioxidant capacity were analyzed. Phenolic compounds were quantified, and anthocyanins were identified by high-performance liquid chromatography coupled to diode-array detection and mass spectrometry (HPLC-DAD-MS). Concentration factors higher than 4.0 were obtained for anthocyanins, gallic acid, and catechin after nanofiltration of the extracts. Other compounds such as epicatechin, p-Coumaric acid, and ferulic acid were quantified in the concentrated extract, and the main anthocyanins identified were 3,5-diglucoside: petunidin, malvidin, and delphinidin. Therefore, jambolan extract showed a high potential to be used as a natural dye and antioxidant in food products.
罗望子是一种富含生物活性化合物的未开发水果,如花色苷、儿茶素和没食子酸。因此,生物活性化合物的提取可以为水果增加附加值。在此背景下,本研究首次报道了通过超滤和纳滤法从罗望子果肉和果皮中回收和浓缩提取物。酸化水用于提取罗望子果肉和果皮中的多酚类物质。采用截留分子量为 180 至 4000g/mol 的超滤和纳滤膜对提取物进行浓缩。分析了总单体花色苷、总酚类化合物和抗氧化能力。采用高效液相色谱-二极管阵列检测-质谱联用(HPLC-DAD-MS)对酚类化合物进行定量,对花色苷进行鉴定。浓缩后花色苷、没食子酸和儿茶素的浓缩因子均高于 4.0。在浓缩提取物中还定量了表儿茶素、对香豆酸和阿魏酸等其他化合物,并鉴定了主要的花色苷为 3,5-二糖苷:矢车菊素、锦葵色素和飞燕草素。因此,罗望子提取物具有作为天然染料和食品抗氧化剂的巨大潜力。