Freitag Hendrik, de Vries Rick, Paterno Marta, Maestri Simone, Delledonne Massimo, Thompson Cameron G, Lamed Helena, Lambert Rebekah, Fox Michael F, Gonzalez Mariela C, Delocado Emmanuel D, Sabordo Marc R, Pangantihon Clister V, Njunjić Iva
Ateneo de Manila University, Quezon City, Philippines Ateneo de Manila University Quezon City Philippines.
Amstelveenseweg 980B, Amsterdam, Netherlands Amstelveenseweg 980B Amsterdam Netherlands.
Biodivers Data J. 2021 Jan 12;9:e59892. doi: 10.3897/BDJ.9.e59892. eCollection 2021.
Long-palped Water Beetles were collected during a taxon expedition in Montenegro which involved citizen scientists, students and taxonomists. The material was collected from springs, brooks, fens and the Tara River, at altitudes between 600 m and 1450 m above sea level, using fine-meshed hand-nets and by manual checking of submerged substrates. The morphological species delimitation was supplemented and congruent with mtDNA sequences mainly obtained in the field using the newly-developed MinION-based pipeline.
The new species Freitag & de Vries, sp. n. from Durmitor Mt. is described, illustrated and compared in detail to closely-related congeners of the d'Orchymont, 1930/ Rey, 1885 species complex. Five additional species and female specimens of two unidentified morphospecies of the genus were also recorded in the vicinity of Durmitor National Park. New records and the first DNA barcodes for Jäch & Díaz, 2012 (endemic to Montenegro) and Kiesenwetter, 1849 are provided. Further records of Germar, 1824, Stephens, 1829, Ganglbauer, 1901 and females of two unidentified morphospecies are commented upon. The resulting inter- and intraspecific genetic distances and some observations of low or zero sequence divergence between recently-diverged species of Kugelann, 1794 are briefly discussed.
长触水龟甲是在黑山的一次分类学考察中收集的,该考察有公民科学家、学生和分类学家参与。材料采集于海拔600米至1450米的泉水、溪流、沼泽和塔拉河,使用细网手网并通过人工检查水下基质来进行。形态学物种界定得到了补充,并且与主要在野外使用新开发的基于MinION的流程获得的线粒体DNA序列一致。
描述了来自杜米托尔山的新物种弗赖塔格 & 德弗里斯,新种,并进行了详细的绘图,还与1930年的多尔希蒙特/1885年的雷伊物种复合体中的近缘同属物种进行了详细比较。在杜米托尔国家公园附近还记录了该属的另外五个物种以及两个未鉴定形态物种的雌性标本。提供了2012年雅赫 & 迪亚兹(黑山特有种)和1849年基森韦特的新记录及首个DNA条形码。对1824年的杰马尔、1829年的斯蒂芬斯、1901年的甘布尔鲍尔以及两个未鉴定形态物种的雌性的进一步记录进行了评论。简要讨论了由此得出的种间和种内遗传距离,以及对1794年库格兰的最近分化物种之间低序列差异或零序列差异的一些观察结果。