Tu Yunjie, Liu Yifan, Zhang Ming, Shan Yanju, Ji Gaige, Ju Xiaojun, Zou Jianmin, Shu Jingting
Poultry Institute, Chinese Academy of Agricultural Sciences, Cangjie road, number 58, Yangzhou, 225125, China.
Key Lab of Poultry Genetics and Breeding in Jiangsu Province, Cangjie road, number 58, Yangzhou, 225125, China.
J Poult Sci. 2021 Jan 25;58(1):5-11. doi: 10.2141/jpsa.0190104.
The aim of this study was to identify genes involved in comb development to provide insights into the molecular mechanism of chickens' comb formation. Fixation index (F) and average number of base differences () of males with large and small combs were calculated based on whole-genome resequencing data. Chromosome regions with larger F values and smaller were considered candidate selection regions. Through further annotation of gene functions and pathways, we sought to screen possible selected genes associated with comb development. By screening whole genome resequencing data, F and were calculated using a 40 Kb sliding window strategy and eight regions were identified. Quantitative trait loci (QTL; gene) related to comb length were found on chromosome 1. QTL (, and genes) related to comb weight were found on chromosome 3. QTL (, and genes) associated with comb area were found on the Z chromosome. Nineteen genes, Wnt signaling pathway and neuroactive ligand-receptor interaction signaling pathway directly or indirectly related to comb growth and development were found through functional annotation and GO analysis. Among the selected genes and Wnt signaling pathways were related to immunity. and neuroactive ligand receptor interaction signaling pathways related to neural function were screened. , and genes were related to heat dissipation. Among the selected genes , and associated with immunity, neurological function, and heat dissipation function coincided with genes affecting the length, weight, and area of the comb. Comprehensive analysis suggested that comb development was due to multiple genes and signaling pathways.
本研究的目的是鉴定参与鸡冠发育的基因，以深入了解鸡鸡冠形成的分子机制。基于全基因组重测序数据计算大鸡冠和小鸡冠雄性的固定指数（F）和平均碱基差异数（）。F值较大且较小的染色体区域被视为候选选择区域。通过对基因功能和通路的进一步注释，我们试图筛选与鸡冠发育相关的可能的选择基因。通过筛选全基因组重测序数据，采用40 Kb滑动窗口策略计算F和，共鉴定出8个区域。在1号染色体上发现了与鸡冠长度相关的数量性状位点（QTL；基因）。在3号染色体上发现了与鸡冠重量相关的QTL（、和基因）。在Z染色体上发现了与鸡冠面积相关的QTL（、和基因）。通过功能注释和GO分析，发现了19个与鸡冠生长发育直接或间接相关的基因、Wnt信号通路和神经活性配体-受体相互作用信号通路。在所选基因中，和Wnt信号通路与免疫有关。筛选出了与神经功能相关的和神经活性配体受体相互作用信号通路。、和基因与散热有关。在所选基因中，与免疫、神经功能和散热功能相关的、和与影响鸡冠长度、重量和面积的基因一致。综合分析表明，鸡冠发育是由多个基因和信号通路引起的。
