Hoover E L, Ross M, Fani K, Webb H, Kirshy D, DiMaio F, Ketosugbo A, Debons A F
Department of Surgery, Brooklyn Veterans Administration Medical Center.
J Vasc Surg. 1988 Apr;7(4):543-8.
Patency of vein grafts may be influenced by the medium in which they are stored temporarily. We compared saline solution vs blood on prostanoid production and maintenance of endothelium in canine veins after 1 hour of storage at 23 degrees C with 0.2 mg/ml of papaverine. Spontaneous and arachidonate-stimulated prostaglandin levels were measured by radioimmunoassay. Endothelial integrity was analyzed by light and electron microscopy. Prostaglandin production in blood vs that in saline solution was 1821 +/- 1264 and 1259 +/- 719 pg/cm2/min at control and 6705 +/- 3702 vs 6264 +/- 3409 pg/cm2/min, respectively, after stimulation. There were no statistically significant differences between the groups at any time point. Thromboxane levels were also indistinguishable between groups. Microscopy revealed 70% endothelial loss in blood vs 95% for saline solution. We conclude that endothelial preservation is enhanced by blood storage, that the medial layer produces substantial amounts of prostacyclin, and that additional storage solutions need to be investigated.
静脉移植物的通畅性可能会受到其临时储存介质的影响。我们比较了在23摄氏度下用0.2毫克/毫升罂粟碱储存1小时后，生理盐水与血液对犬类静脉中前列腺素生成和内皮维持的影响。通过放射免疫测定法测量自发和花生四烯酸刺激的前列腺素水平。通过光学显微镜和电子显微镜分析内皮完整性。在对照时，血液中与生理盐水中的前列腺素生成分别为1821±1264和1259±719皮克/平方厘米/分钟，刺激后分别为6705±3702和6264±3409皮克/平方厘米/分钟。在任何时间点，两组之间均无统计学上的显著差异。血栓素水平在两组之间也无差异。显微镜检查显示，血液中的内皮损失为70%，而生理盐水为95%。我们得出结论，血液储存可增强内皮保存，中层产生大量前列环素，并且需要研究其他储存溶液。
