Gathercole Jessica L, Grosvenor Anita J, Lee Erin, Thomas Ancy, Mitchell Cameron J, Zeng Nina, D'Souza Randall F, Ramzan Farha, Sharma Pankaja, Knowles Scott O, Roy Nicole C, Sjödin Anders, Wagner Karl-Heinz, Milan Amber M, Mitchell Sarah M, Cameron-Smith David
Proteins and Metabolites Team, AgResearch, Lincoln, Christchurch, New Zealand.
School of Kinesiology, University of British Columbia, Vancouver, BC, Canada.
Front Nutr. 2021 Jan 13;7:595905. doi: 10.3389/fnut.2020.595905. eCollection 2020.
Faecal proteomics targeting biomarkers of immunity and inflammation have demonstrated clinical application for the identification of changes in gastrointestinal function. However, there are limited comprehensive analyses of the host faecal proteome and how it may be influenced by dietary factors. To examine this, the post-diet proteome of older males was analysed at the completion of a 10-week dietary intervention, either meeting the minimum dietary protein recommendations (RDA; = 9) or twice the recommended dietary allowance (2RDA, = 10). The host faecal proteome differed markedly between individuals, with only a small subset of proteins present in ≥ 60% of subjects (14 and 44 proteins, RDA and 2RDA, respectively, with only 7 common to both groups). No differences were observed between the diet groups on the profiles of host faecal proteins. Faecal proteins were detected from a wide range of protein classes, with high inter-individual variation and absence of obvious impact in response to diets with markedly different protein intake. This suggests that well-matched whole food diets with two-fold variation in protein intake maintained for 10 weeks have minimal impact on human faecal host proteins.
针对免疫和炎症生物标志物的粪便蛋白质组学已在识别胃肠功能变化方面显示出临床应用价值。然而，对于宿主粪便蛋白质组及其可能如何受饮食因素影响的综合分析却很有限。为了对此进行研究，在一项为期10周的饮食干预结束时，对老年男性的饮食后蛋白质组进行了分析，该饮食干预分为达到最低饮食蛋白质推荐量（RDA；n = 9）或两倍推荐饮食摄入量（2RDA，n = 10）两组。个体之间的宿主粪便蛋白质组存在显著差异，只有一小部分蛋白质在≥60%的受试者中存在（分别为14种和44种蛋白质，RDA组和2RDA组，两组仅有7种蛋白质相同）。在宿主粪便蛋白质谱方面，饮食组之间未观察到差异。从广泛的蛋白质类别中检测到了粪便蛋白质，但个体间差异很大，且在蛋白质摄入量明显不同的饮食反应中没有明显影响。这表明，蛋白质摄入量有两倍差异的搭配良好的全食物饮食持续10周，对人类粪便宿主蛋白质的影响最小。
