Suppr超能文献

工程化胰岛/脂肪来源间充质干细胞片皮下移植于全胰切除的糖尿病猪体内。

Subcutaneous transplantation of engineered islet/adipose-derived mesenchymal stem cell sheets in diabetic pigs with total pancreatectomy.

作者信息

Yamashita Mampei, Adachi Toshiyuki, Adachi Tomohiko, Ono Shinichiro, Matsumura Naomi, Maekawa Kyoichiro, Sakai Yusuke, Hidaka Masaaki, Kanetaka Kengo, Kuroki Tamotsu, Eguchi Susumu

机构信息

Department of Surgery, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki city, Nagasaki, 852-8501, Japan.

Department of Surgery, National Hospital Organization Nagasaki Medical Center, 2-1001-1 Kubara, Omura city, Nagasaki, 856-8562, Japan.

出版信息

Regen Ther. 2021 Jan 13;16:42-52. doi: 10.1016/j.reth.2020.12.005. eCollection 2021 Mar.

Abstract

INTRODUCTION

Intraportal islet transplantation is a promising therapeutic approach for patients with type 1 diabetes mellitus (T1DM). However, despite being minimally invasive, the method has some limitations, such as short-term graft loss, portal venous thrombosis, and difficulty in collecting adequate amounts of islets. Subcutaneous islet transplantation on adipose-derived mesenchymal stem cell (ADSC) sheets has been suggested to overcome these limitations, and in this study, we have examined its feasibility in T1DM pigs.

METHODS

Inguinal subcutaneous fat was harvested from young pigs and then isolated and cultured adequate ADSCs to prepare sheets. Islets were isolated from the pancreases of mature pigs and seeded on the ADSC sheets. T1DM pigs were generated by total pancreatectomy, and ADSC sheets with transplanted islets were administered subcutaneously to the waist ( = 2). The effects of the islets on the ADSC sheets and on blood glucose levels were evaluated. Insulin secretion was measured by insulin stimulation index.

RESULTS

Islet viability was higher on ADSCs compared to islets alone (91.8 ± 4.3 vs. 81.7 ± 4.1%). The insulin stimulation index revealed higher glucose sensitivity of islets on ADSC sheets compared to islets alone (2.8 ± 2.0 vs. 0.8 ± 0.3). After transplantation, the blood glucose levels of two pigs were within the normal range, and sensitive insulin secretion was confirmed by intravenous glucose tolerance tests. After graftectomy, decreased insulin secretion and hyperglycemia were observed.

CONCLUSIONS

Subcutaneous islet transplantation using ADSC sheets can regulate the blood glucose levels of T1DM pigs.

摘要

引言

门静脉内胰岛移植是1型糖尿病(T1DM)患者一种很有前景的治疗方法。然而,尽管该方法具有微创性,但仍存在一些局限性,如短期移植物丢失、门静脉血栓形成以及难以收集足够数量的胰岛。有人提出在脂肪来源的间充质干细胞(ADSC)片上进行皮下胰岛移植可克服这些局限性,在本研究中,我们检测了其在T1DM猪中的可行性。

方法

从幼猪腹股沟处采集皮下脂肪,然后分离并培养足够数量的ADSC以制备细胞片。从成年猪的胰腺中分离胰岛,并接种到ADSC片上。通过全胰切除术制备T1DM猪,将移植了胰岛的ADSC片皮下注射到腰部(n = 2)。评估胰岛在ADSC片上的效果以及对血糖水平的影响。通过胰岛素刺激指数测量胰岛素分泌。

结果

与单独的胰岛相比,ADSC上的胰岛活力更高(91.8±4.3%对81.7±4.1%)。胰岛素刺激指数显示,与单独的胰岛相比,ADSC片上的胰岛对葡萄糖的敏感性更高(2.8±2.0对0.8±0.3)。移植后,两只猪的血糖水平在正常范围内,静脉葡萄糖耐量试验证实胰岛素分泌敏感。移植切除后,观察到胰岛素分泌减少和高血糖。

结论

使用ADSC片进行皮下胰岛移植可调节T1DM猪的血糖水平。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b57/7810917/4c04a2adefe6/gr1.jpg

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验