Nguyen Nhung H A, Špánek Roman, Falagan-Lotsch Priscila, Ševců Alena
Institute for Nanomaterials, Advanced Technologies and Innovation, Technical University of Liberec (TUL), Studentská 2, 46117, Liberec, Czech Republic.
Faculty of Mechatronics, Informatics and Interdisciplinary Studies, Technical University of Liberec (TUL), Studentská 2, 46117, Liberec, Czech Republic.
Curr Microbiol. 2021 Mar;78(3):979-991. doi: 10.1007/s00284-021-02362-7. Epub 2021 Feb 1.
The application of zero-valent iron particles (ZVI) for the treatment of heavily polluted environment and its biological effects have been studied for at least two decades. Still, information on the impact on bacterial metabolic pathways is lacking. This study describes the effect of microscale and nanoscale ZVI (mZVI and nZVI) on the abundance of different metabolic pathways in freshwater bacterial communities. The metabolic pathways were inferred from metabolism modelling based on 16S rRNA gene sequence data using paprica pipeline. The nZVI changed the abundance of numerous metabolic pathways compared to a less influencing mZVI. We identified the 50 most affected pathways, where 31 were related to degradation, 17 to biosynthesis, and 2 to detoxification. The linkage between pathways was two times higher in nZVI samples compared to mZVI, and was specifically higher considering the arsenate detoxification II pathway. Limnohabitans and Roseiflexus were linked to the same pathways in both nZVI and mZVI. The prediction of metabolic pathways increases our knowledge of the impacts of nZVI and mZVI on freshwater bacterioplankton.
零价铁颗粒（ZVI）在重度污染环境治理中的应用及其生物学效应已被研究了至少二十年。然而，关于其对细菌代谢途径影响的信息仍然匮乏。本研究描述了微米级和纳米级ZVI（mZVI和nZVI）对淡水细菌群落中不同代谢途径丰度的影响。代谢途径是通过基于16S rRNA基因序列数据，使用paprica管道进行代谢建模推断得出的。与影响较小的mZVI相比，nZVI改变了众多代谢途径的丰度。我们确定了受影响最大的50条途径，其中31条与降解有关，17条与生物合成有关，2条与解毒有关。与mZVI相比，nZVI样品中途径之间的联系高出两倍，特别是考虑到砷酸盐解毒II途径时更高。在nZVI和mZVI中，Limnohabitans和Roseiflexus与相同的途径相关。代谢途径的预测增加了我们对nZVI和mZVI对淡水浮游细菌影响的认识。
