Department of Food Science and Human Wellness, Rakuno Gakuen University, Ebetsu, Japan.
Research Office Concerning the Health of Humans and Birds, Abashiri, Japan.
Anim Sci J. 2021 Jan-Dec;92(1):e13517. doi: 10.1111/asj.13517.
Lipofuscin is one of the indicators of oxidative stress. To elucidate the role of oxidative stress in the development of wooden breast, this study investigates lipofuscin accumulation in various parts of wooden breast muscles. Sampling was performed using 46-day-old broiler chickens housed at a commercial Japanese poultry slaughterhouse. Fourteen wooden breast fillets and 13 normal breast fillets were collected in the deboning line. The samples used to measure shear force, 2-thiobarbituric acid reactive substance (TBARS), and for histological analysis were taken from the six portions of breast muscle fillets. In muscles affected by wooden breast, vacuolated muscle fibers were observed, and connective tissues appearing like perimysium were expanded with fibrosis. TBARS value and accumulation of lipofuscin were significantly higher in the wooden breast than in the normal breasts. A lot of lipofuscin granules were localized in the cytoplasm of collapsed muscle fibers of the wooden breast. The cranial portion of the wooden breast showed the highest shear force. The cranial position had a large amount of connective tissue and lipofuscin granules. The results of the present study strongly suggest that high oxidative stress, especially with a significant accumulation of lipofuscin, is associated with the development of wooden breasts.
脂褐素是氧化应激的指标之一。为了阐明氧化应激在木质鸡胸肉形成中的作用，本研究调查了木质鸡胸肉各部位脂褐素的积累情况。研究使用了来自商业日本家禽屠宰场的 46 日龄肉鸡进行采样。在去骨线上收集了 14 块木质鸡胸肉片和 13 块正常鸡胸肉片。用于测量剪切力、2-硫代巴比妥酸反应物（TBARS）和组织学分析的样品取自六个鸡胸肉片部位。在受木质鸡胸肉影响的肌肉中，观察到空泡化的肌纤维，并且类似于肌间膜的结缔组织扩张伴有纤维化。木质鸡胸肉中的 TBARS 值和脂褐素积累明显高于正常鸡胸肉。大量脂褐素颗粒定位于木质鸡胸肉中塌陷的肌纤维细胞质中。木质鸡胸肉的头部剪切力最高。头部位置有大量的结缔组织和脂褐素颗粒。本研究的结果强烈表明，高氧化应激，特别是脂褐素的大量积累，与木质鸡胸肉的发展有关。
