重组腺相关病毒 8 介导的猪圆环病毒 2 衣壳蛋白的表达与评估。

Expression and evaluation of porcine circovirus type 2 capsid protein mediated by recombinant adeno-associated virus 8.

机构信息

National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun 130012, China.

School of Chemistry and Life Science, Changchun University of Technology, Changchun 130012, China.

出版信息

J Vet Sci. 2021 Jan;22(1):e8. doi: 10.4142/jvs.2021.22.e8.

BACKGROUND

Porcine circovirus type 2 (PCV2) is an important infectious pathogen implicated in porcine circovirus-associated diseases (PCVAD), which has caused significant economic losses in the pig industry worldwide.

OBJECTIVES

A suitable viral vector-mediated gene transfer platform for the expression of the capsid protein (Cap) is an attractive strategy.

METHODS

In the present study, a recombinant adeno-associated virus 8 (rAAV8) vector was constructed to encode Cap (Cap-rAAV) and after gene transfer.

RESULTS

The obtained results showed that Cap could be expressed in HEK293T cells and BABL/c mice. The results of lymphocytes proliferative, as well as immunoglobulin G (IgG) 2a and interferon-γ showed strong cellular immune responses induced by Cap-rAAV. The enzyme-linked immunosorbent assay titers obtained and the IgG1 and interleukin-4 levels showed that humoral immune responses were also induced by Cap-rAAV. Altogether, these results demonstrated that the rAAV8 vaccine Cap-rAAV can induce strong cellular and humoral immune responses, indicating a potential rAAV8 vaccine against PCV2.

CONCLUSIONS

The injection of rAAV8 encoding PCV2 Cap genes into muscle tissue can ensure long-term, continuous, and systemic expression.

背景

猪圆环病毒 2 型（PCV2）是一种重要的传染性病原体，与猪圆环病毒相关疾病（PCVAD）有关，它在世界范围内的养猪业造成了重大的经济损失。

目的

合适的病毒载体介导的基因转移平台对于衣壳蛋白（Cap）的表达是一种有吸引力的策略。

方法

在本研究中，构建了一种重组腺相关病毒 8（rAAV8）载体，用于编码 Cap（Cap-rAAV），并进行基因转移。

结果

结果表明，Cap 可以在 HEK293T 细胞和 BABL/c 小鼠中表达。淋巴细胞增殖以及免疫球蛋白 G（IgG）2a 和干扰素-γ的结果表明，Cap-rAAV 诱导了强烈的细胞免疫反应。酶联免疫吸附试验滴度和 IgG1 和白细胞介素-4 水平的结果表明，Cap-rAAV 也诱导了体液免疫反应。总之，这些结果表明，rAAV8 疫苗 Cap-rAAV 可以诱导强烈的细胞和体液免疫反应，表明它是一种针对 PCV2 的潜在 rAAV8 疫苗。