Wils E R, Hulst A G, van Laar J
Prins Maurits Laboratory, TNO, Rijswijk, The Netherlands.
J Anal Toxicol. 1988 Jan-Feb;12(1):15-9. doi: 10.1093/jat/12.1.15.
Improvements on a procedure for the determination of thiodiglycol in urine are presented. This procedure is based on the conversion of thiodiglycol to mustard gas with concentrated HCl followed by headspace analysis. With deuterated thiodiglycol as the internal standard, more accurate quantitative analyses are possible. Residual amounts of chlorine in the water used for preparation of standard solutions posed problems, and the reaction between chlorine and thiodiglycol in water has been studied. The possible formation of mustard gas from thiodiglycol and sodium chloride was also investigated. The modified procedure was applied to urine samples of several Iranian patients who were victims of an alleged attack with mustard gas and who were treated in European hospitals in 1986. With the exception of one relatively high value (330 ng/mL), the thiodiglycol concentrations were in the same range (10 to 100 ng/mL) as those found during an investigation in 1984. The urine of 20 male controls contained thiodiglycol amounts not above 20 ng/mL. The combined data obtained in 1984 and 1986 (25 Iranian patients and 25 controls) show statistically significant differences. Approximately 80% of the Iranian patients had levels above the 95% confidence limit calculated from the control group.
本文介绍了尿中硫二甘醇测定方法的改进。该方法基于用浓盐酸将硫二甘醇转化为芥子气，然后进行顶空分析。以氘代硫二甘醇作为内标，可以进行更准确的定量分析。制备标准溶液所用水中的残留氯量带来了问题，因此对水中氯与硫二甘醇之间的反应进行了研究。还研究了硫二甘醇与氯化钠生成芥子气的可能性。改进后的方法应用于1986年在欧洲医院接受治疗的几名据称遭受芥子气袭击的伊朗患者的尿液样本。除了一个相对较高的值（330纳克/毫升）外，硫二甘醇浓度与1984年调查期间发现的浓度处于同一范围（10至100纳克/毫升）。20名男性对照者的尿液中硫二甘醇含量不超过20纳克/毫升。1984年和1986年获得的综合数据（25名伊朗患者和25名对照者）显示出统计学上的显著差异。大约80%的伊朗患者的水平高于根据对照组计算出的95%置信限。
