金黄地鼠感染 SARS-CoV-2 后的嗅上皮再生特征。
Regeneration Profiles of Olfactory Epithelium after SARS-CoV-2 Infection in Golden Syrian Hamsters.
机构信息
Department of Otolaryngology, University of Texas Medical Branch, Galveston, Texas 77555, United States.
Department of Otolaryngology, Graduate School of Medicine, The University of Tokyo, Tokyo 113-0033, Japan.
出版信息
ACS Chem Neurosci. 2021 Feb 17;12(4):589-595. doi: 10.1021/acschemneuro.0c00649. Epub 2021 Feb 1.
Abstract
Olfactory dysfunction is one of the most frequent and specific symptoms of coronavirus disease 2019 (COVID-19). Information on the damage and repair of the neuroepithelium and its impact on olfactory function after COVID-19 is still incomplete. While severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) causes the ongoing worldwide outbreak of COVID-19, little is known about the changes triggered by SARS-CoV-2 in the olfactory epithelium (OE) at the cellular level. Here, we report profiles of the OE after SARS-CoV-2 infection in golden Syrian hamsters, which is a reliable animal model of COVID-19. We observed severe damage in the OE as early as 3 days postinoculation and regionally specific damage and regeneration of the OE within the nasal cavity; the nasal septal region demonstrated the fastest recovery compared to other regions in the nasal turbinates. These findings suggest that anosmia related to SARS-CoV-2 infection may be fully reversible.
摘要
嗅觉功能障碍是 2019 冠状病毒病（COVID-19）最常见和最具特异性的症状之一。有关 COVID-19 后神经上皮损伤和修复及其对嗅觉功能影响的信息尚不完全。虽然严重急性呼吸综合征冠状病毒 2（SARS-CoV-2）导致了目前正在全球范围内爆发的 COVID-19，但人们对 SARS-CoV-2 在嗅觉上皮（OE）细胞水平上引发的变化知之甚少。在这里，我们报告了金黄地鼠感染 SARS-CoV-2 后 OE 的情况，金黄地鼠是 COVID-19 的可靠动物模型。我们观察到早在接种后 3 天，OE 就受到严重损伤，鼻腔内 OE 出现区域特异性损伤和再生；与鼻甲的其他区域相比，鼻中隔区域的恢复最快。这些发现表明，与 SARS-CoV-2 感染相关的嗅觉丧失可能是完全可逆的。
相似文献
1
Regeneration Profiles of Olfactory Epithelium after SARS-CoV-2 Infection in Golden Syrian Hamsters.金黄地鼠感染 SARS-CoV-2 后的嗅上皮再生特征。
ACS Chem Neurosci. 2021 Feb 17;12(4):589-595. doi: 10.1021/acschemneuro.0c00649. Epub 2021 Feb 1.
2
Massive transient damage of the olfactory epithelium associated with infection of sustentacular cells by SARS-CoV-2 in golden Syrian hamsters.大剂量的嗅上皮细胞暂时性损伤与 SARS-CoV-2 感染叙利亚金黄仓鼠的支持细胞有关。
Brain Behav Immun. 2020 Oct;89:579-586. doi: 10.1016/j.bbi.2020.06.032. Epub 2020 Jul 3.
3
The Microvillar and Solitary Chemosensory Cells as the Novel Targets of Infection of SARS-CoV-2 in Syrian Golden Hamsters.《叙利亚金黄地鼠中新型冠状病毒 2 感染的微绒毛和孤立化学感觉细胞作为新靶点》。
Viruses. 2021 Aug 20;13(8):1653. doi: 10.3390/v13081653.
4
COVID-19 Anosmia: High Prevalence, Plural Neuropathogenic Mechanisms, and Scarce Neurotropism of SARS-CoV-2?COVID-19 嗅觉丧失：SARS-CoV-2 的高患病率、多种神经发病机制和很少的神经嗜性？
Viruses. 2021 Nov 4;13(11):2225. doi: 10.3390/v13112225.
5
Recovery of anosmia in hamsters infected with SARS-CoV-2 is correlated with repair of the olfactory epithelium.感染 SARS-CoV-2 的仓鼠的嗅觉丧失恢复与嗅上皮修复有关。
Sci Rep. 2022 Jan 12;12(1):628. doi: 10.1038/s41598-021-04622-9.
6
COVID-19-related anosmia is associated with viral persistence and inflammation in human olfactory epithelium and brain infection in hamsters.与COVID-19相关的嗅觉丧失与人类嗅觉上皮中的病毒持续存在和炎症以及仓鼠的脑部感染有关。
Sci Transl Med. 2021 Jun 2;13(596). doi: 10.1126/scitranslmed.abf8396. Epub 2021 May 3.
7
Severe Acute Respiratory Syndrome Coronavirus 2 Infects and Damages the Mature and Immature Olfactory Sensory Neurons of Hamsters.严重急性呼吸综合征冠状病毒 2 感染并损伤仓鼠成熟和未成熟的嗅觉感觉神经元。
Clin Infect Dis. 2021 Jul 15;73(2):e503-e512. doi: 10.1093/cid/ciaa995.
8
The Molecular Basis of Olfactory Dysfunction in COVID-19 and Long COVID.新冠病毒和长新冠嗅觉功能障碍的分子基础。
Lifestyle Genom. 2024;17(1):42-56. doi: 10.1159/000539292. Epub 2024 May 15.
9
Oral SARS-CoV-2 Inoculation Causes Nasal Viral Infection Leading to Olfactory Bulb Infection: An Experimental Study.经口接种 SARS-CoV-2 可导致鼻腔病毒感染进而引起嗅球感染：一项实验研究。
Front Cell Infect Microbiol. 2022 Jun 13;12:924725. doi: 10.3389/fcimb.2022.924725. eCollection 2022.
10
Olfactory Cleft Measurements and COVID-19-Related Anosmia.嗅裂测量与 COVID-19 相关嗅觉障碍。
Otolaryngol Head Neck Surg. 2021 Jun;164(6):1337-1344. doi: 10.1177/0194599820965920. Epub 2020 Oct 13.
引用本文的文献
1
The SARS-CoV-2 envelope PDZ binding motif acts as a virulence factor disrupting host's epithelial cell-cell junctions.严重急性呼吸综合征冠状病毒2（SARS-CoV-2）包膜蛋白的PDZ结合基序作为一种毒力因子，破坏宿主上皮细胞间连接。
Cell Mol Biol Lett. 2025 Jul 11;30(1):80. doi: 10.1186/s11658-025-00758-y.
2
Histopathology of the tongue in a hamster model of COVID-19.新冠病毒病仓鼠模型中舌部的组织病理学
BMC Oral Health. 2025 Jan 23;25(1):121. doi: 10.1186/s12903-025-05420-9.
3
Histopathology of the Tongue in a Hamster Model of COVID-19.新冠病毒肺炎仓鼠模型中舌部的组织病理学
Res Sq. 2024 Jul 4:rs.3.rs-4590482. doi: 10.21203/rs.3.rs-4590482/v1.
4
Inflammatory Response and Defects on Myelin Integrity in the Olfactory System of K18hACE2 Mice Infected with SARS-CoV-2.感染 SARS-CoV-2 的 K18hACE2 小鼠嗅系统中的炎症反应和髓鞘完整性缺陷。
eNeuro. 2024 Jun 17;11(6). doi: 10.1523/ENEURO.0106-24.2024. Print 2024 Jun.
5
SARS-CoV-2 and Other Respiratory Viruses in Human Olfactory Pathophysiology.严重急性呼吸综合征冠状病毒2（SARS-CoV-2）及其他呼吸道病毒在人类嗅觉病理生理学中的作用
Microorganisms. 2024 Mar 7;12(3):540. doi: 10.3390/microorganisms12030540.
6
Mechanisms of COVID-19-associated olfactory dysfunction.COVID-19 相关嗅觉功能障碍的发病机制。
Neuropathol Appl Neurobiol. 2024 Apr;50(2):e12960. doi: 10.1111/nan.12960.
7
Vaccination against SARS-CoV-2 Does Not Protect against the Development of Anosmia in a Hamster Model.在仓鼠模型中，接种抗SARS-CoV-2疫苗不能预防嗅觉丧失的发生。
Vaccines (Basel). 2023 Oct 5;11(10):1564. doi: 10.3390/vaccines11101564.
8
The Olfactory System: Basic Anatomy and Physiology for General Otorhinolaryngologists.嗅觉系统：普通耳鼻咽喉科医生的基础解剖与生理学
Clin Exp Otorhinolaryngol. 2023 Nov;16(4):308-316. doi: 10.21053/ceo.2023.00185. Epub 2023 Sep 4.
9
Neuroinvasion and anosmia are independent phenomena upon infection with SARS-CoV-2 and its variants.神经侵袭和嗅觉丧失是感染 SARS-CoV-2 及其变体时独立的现象。
Nat Commun. 2023 Jul 26;14(1):4485. doi: 10.1038/s41467-023-40228-7.
10
Mechanisms of SARS-CoV-2-associated anosmia.SARS-CoV-2 相关嗅觉丧失的机制。
Physiol Rev. 2023 Oct 1;103(4):2759-2766. doi: 10.1152/physrev.00012.2023. Epub 2023 Jun 21.
本文引用的文献
1
ACE2, TMPRSS2, and Furin expression in the nose and olfactory bulb in mice and humans.ACE2、TMPRSS2 和 Furin 在小鼠和人类鼻腔及嗅球中的表达。
Rhinology. 2021 Feb 1;59(1):105-109. doi: 10.4193/Rhin20.324.
2
Chemosensory Dysfunction in COVID-19: Integration of Genetic and Epidemiological Data Points to D614G Spike Protein Variant as a Contributing Factor.新冠病毒感染相关的嗅觉味觉障碍：遗传与流行病学数据的整合提示 D614G 棘突蛋白变异可能是致病因素之一。
ACS Chem Neurosci. 2020 Oct 21;11(20):3180-3184. doi: 10.1021/acschemneuro.0c00596. Epub 2020 Sep 30.
3
Non-neuronal expression of SARS-CoV-2 entry genes in the olfactory system suggests mechanisms underlying COVID-19-associated anosmia.SARS-CoV-2 进入基因在嗅觉系统中的非神经元表达提示了 COVID-19 相关嗅觉丧失的潜在机制。
Sci Adv. 2020 Jul 31;6(31). doi: 10.1126/sciadv.abc5801. Epub 2020 Jul 24.
4
Individual Differences in Thresholds and Consumer Preferences for Rotundone Added to Red Wine.葡萄酒中添加橡木香轮烷对阈值和消费者偏好的个体差异。
Nutrients. 2020 Aug 20;12(9):2522. doi: 10.3390/nu12092522.
5
A systematic review of neurological symptoms and complications of COVID-19.一项关于 COVID-19 的神经系统症状和并发症的系统评价。
J Neurol. 2021 Feb;268(2):392-402. doi: 10.1007/s00415-020-10067-3. Epub 2020 Jul 20.
6
Massive transient damage of the olfactory epithelium associated with infection of sustentacular cells by SARS-CoV-2 in golden Syrian hamsters.大剂量的嗅上皮细胞暂时性损伤与 SARS-CoV-2 感染叙利亚金黄仓鼠的支持细胞有关。
Brain Behav Immun. 2020 Oct;89:579-586. doi: 10.1016/j.bbi.2020.06.032. Epub 2020 Jul 3.
7
Olfactory and Gustatory Outcomes in COVID-19: A Prospective Evaluation in Nonhospitalized Subjects.COVID-19 患者的嗅觉和味觉结果：非住院患者的前瞻性评估。
Otolaryngol Head Neck Surg. 2020 Dec;163(6):1144-1149. doi: 10.1177/0194599820939538. Epub 2020 Jun 30.
8
Persisting olfactory dysfunction in patients after recovering from COVID-19.新冠病毒感染康复后患者持续存在嗅觉功能障碍。
J Infect. 2020 Sep;81(3):e58. doi: 10.1016/j.jinf.2020.06.054. Epub 2020 Jun 24.
9
Syrian hamsters as a small animal model for SARS-CoV-2 infection and countermeasure development.叙利亚仓鼠作为 SARS-CoV-2 感染及对策研发的小动物模型。
Proc Natl Acad Sci U S A. 2020 Jul 14;117(28):16587-16595. doi: 10.1073/pnas.2009799117. Epub 2020 Jun 22.
10
Neuropathological Features of Covid-19.新冠病毒病的神经病理学特征
N Engl J Med. 2020 Sep 3;383(10):989-992. doi: 10.1056/NEJMc2019373. Epub 2020 Jun 12.
Zotero 插件