Schwarz Marek, Vohradský Jiří, Pánek Josef
Laboratory of Bioinformatics, Institute of Microbiology, The Czech Academy of Sciences, Praha, Czech Republic.
Bioinformatics. 2021 Sep 9;37(17):2755-2756. doi: 10.1093/bioinformatics/btab073.
We present a web service for improving characterization of non-coding RNAs (ncRNAs) from NCBI BLAST outputs, based on a command-line application rboAnalyzer. Briefly, the application extends subject sequences of selected high scoring pairs (HSPs) in BLAST output to their plausible full length, and predicts their homology and secondary structures. The aim of the application is to aid to characterize subject RNAs in HSPs that come uncharacterized in BLAST output. The main advantages of the web-server are easy use and interactive analysis with search, filtering and data export options.
The web server is freely available at rboanalyzer.elixir-czech.cz. The website frontend is implemented in Elm, while backend is implemented in Python and served by Apache.
我们基于命令行应用程序rboAnalyzer展示了一种用于改进从NCBI BLAST输出中对非编码RNA（ncRNA）进行特征描述的网络服务。简而言之，该应用程序将BLAST输出中选定的高分对（HSP）的主题序列扩展到其合理的全长，并预测它们的同源性和二级结构。该应用程序的目的是帮助对BLAST输出中未表征的HSP中的主题RNA进行特征描述。该网络服务器的主要优点是易于使用以及具有搜索、过滤和数据导出选项的交互式分析。
该网络服务器可在rboanalyzer.elixir-czech.cz上免费获取。网站前端用Elm实现，而后端用Python实现并由Apache提供服务。
