NCR3LG1（B7-H6）是膀胱癌患者的一个潜在预后因素。

NCR3LG1 (B7-H6) is a potential prognostic factor for bladder cancer patients.

机构信息

Tianjin Institute of Urology, The Second Hospital of Tianjin Medical University, Tianjin, China.

Department of Urology, Tianjin Institute of Urology, The Second Hospital of Tianjin Medical University, Tianjin, China.

出版信息

Biomarkers. 2021 May;26(3):260-267. doi: 10.1080/1354750X.2021.1883110. Epub 2021 Feb 10.

DOI:10.1080/1354750X.2021.1883110

PMID:33523715

Abstract

BACKGROUND

NCR3LG1 (B7-H6) protein is selectively overexpressed on tumour and is associated with fatal disease progression of various cancer. However, its prognostic value in bladder cancer (BCa) has not been well elaborated.

METHODS

We examined the expression of NCR3LG1 in human BCa and analysed its clinical significance and prognostic value. Meanwhile, the expression of NCR3LG1 was intervened in human BCa cell line 253JBV to analyse subsequent effects on tumour.

RESULTS

According to TCGA data, the disease-free survival rate was statistically significant between the NCR3LG1 high expression group and the low expression group (Log Rank = 0.006). Immunohistochemical staining showed that the expression of NCR3LG1 in BCa tissue was significantly higher than that in adjacent tissues ( < 0.0001), which was positively correlated with TNM staging ( = 0.008), histological grade ( = 0.022), and lymphoma metastasis of BCa ( = 0.032). The proliferation ( < 0.0001), invasion ( < 0.001) and migration ability ( < 0.001) of 253JBV cells are significantly inhibited by knocking down the expression of NCR3LG1, and the cell cycle arrest is induced at the G1 phase, which accelerates the apoptosis of BCa cells ( < 0.005).

CONCLUSION

Our findings indicate that NCR3LG1 is involved in the progression of human BCa and may become a potential prognostic biomarker for BCa.

摘要

背景

NCR3LG1（B7-H6）蛋白在肿瘤上选择性过表达，与各种癌症的致命疾病进展有关。然而，其在膀胱癌（BCa）中的预后价值尚未得到充分阐述。

方法

我们检测了 NCR3LG1 在人膀胱癌中的表达，并分析了其临床意义和预后价值。同时，我们在人膀胱癌细胞系 253JBV 中干预了 NCR3LG1 的表达，以分析后续对肿瘤的影响。

结果

根据 TCGA 数据，NCR3LG1 高表达组和低表达组之间的无病生存率有统计学意义（Log Rank = 0.006）。免疫组织化学染色显示，NCR3LG1 在膀胱癌组织中的表达明显高于相邻组织（ < 0.0001），与 TNM 分期（ = 0.008）、组织学分级（ = 0.022）和膀胱癌的淋巴瘤转移（ = 0.032）呈正相关。敲低 NCR3LG1 的表达显著抑制了 253JBV 细胞的增殖（ < 0.0001）、侵袭（ < 0.001）和迁移能力（ < 0.001），并诱导细胞周期在 G1 期停滞，加速 BCa 细胞的凋亡（ < 0.005）。