Castro D J, Saxton R E, Fetterman H R, Castro D J, Ward P H
Division of Head and Neck Surgery, UCLA School of Medicine 90024.
Laryngoscope. 1988 Apr;98(4):369-76. doi: 10.1288/00005537-198804000-00002.
Laser photodynamic therapy of superficial malignancies is a promising new approach that will become clinically useful when fluorochromes with high tumor specificity and low toxicity to normal tissues are identified. We recently reported that the mitochondrial dye, Rhodamine-123 (Rh-123), at nontoxic doses, is an effective sensitizing agent for argon laser treatment of human squamous carcinoma and melanoma cells in vitro. We now report the complete inhibition of in vivo tumor development by human M24 melanoma cells transplanted subcutaneously into nu/nu mice after exposure to 1 microgram/ml of Rh-123 for 1 hour and treatment with an argon laser at nonthermal temperatures of 36 to 40 degrees C. Significant in vivo growth was observed for all control tumors. These results demonstrate that Rh-123 enhances the tumoricidal effects of the argon laser at nonthermal temperatures and provides evidence that effective photodynamic therapy may be possible in vivo with the new fluorochrome Rhodamine-123.
浅表恶性肿瘤的激光光动力疗法是一种很有前景的新方法，当鉴定出对正常组织具有高肿瘤特异性和低毒性的荧光染料时，它将在临床上发挥作用。我们最近报道，无毒剂量的线粒体染料罗丹明 - 123（Rh - 123）是氩激光体外治疗人鳞状细胞癌和黑色素瘤细胞的有效敏化剂。我们现在报告，将人M24黑色素瘤细胞皮下移植到裸鼠体内后，在暴露于1微克/毫升的Rh - 123 1小时并用氩激光在36至40摄氏度的非热温度下进行治疗后，体内肿瘤发展完全受到抑制。所有对照肿瘤均观察到显著的体内生长。这些结果表明，Rh - 123增强了氩激光在非热温度下的杀肿瘤作用，并提供了证据表明使用新型荧光染料罗丹明 - 123在体内进行有效的光动力疗法可能是可行的。
