原位接枝温敏水凝胶作为一种双功能固相微萃取涂层，用于可调节生物大分子的萃取。
In-situ grafting temperature-responsive hydrogel as a bifunctional solid-phase microextraction coating for tunable extraction of biomacromolecules.
机构信息
State Key Laboratory of Heavy Oil Processing, Center for Bioengineering and Biotechnology, China University of Petroleum (East China), Qingdao 266580, PR China.
State Key Laboratory of Heavy Oil Processing, Center for Bioengineering and Biotechnology, China University of Petroleum (East China), Qingdao 266580, PR China.
出版信息
J Chromatogr A. 2021 Feb 22;1639:461928. doi: 10.1016/j.chroma.2021.461928. Epub 2021 Jan 21.
A temperature-responsive solid-phase microextraction (SPME) coating was prepared via in-situ atom transfer radical polymerization (ATRP) method. By controlling the temperature of solution below and above the lower critical solution temperature (LCST) of the coating, it can switch between hydrophilic and hydrophobic, thus providing a convenient approach for the selective extraction of analytes with different polarities. The average extraction amount of temperature-responsive coating for polar analytes is about 1.5-fold to that of non-polar ones below LCST, and vice versa. Effective extraction of three biomacromolecules was also obtained by controlling the temperature below or above LCST. The adsorption capacity of the coating for the hydrophilic biomacromolecules at 15 °C is 1.5-2 folds that of 50 °C, whereas the adsorption capacity of the coating to BSA at 50 °C is about 3 folds that of 15 °C. This approach holds great promise for SPME because it provides a simple strategy to prepare bifunctional coatings for various applications.
一种温度响应型固相微萃取（SPME）涂层通过原位原子转移自由基聚合（ATRP）方法制备。通过控制溶液温度低于和高于涂层的低临界溶液温度（LCST），它可以在亲水性和疏水性之间切换，从而为选择性萃取具有不同极性的分析物提供了一种方便的方法。在 LCST 以下，温度响应涂层对极性分析物的平均萃取量约为非极性分析物的 1.5 倍，反之亦然。通过控制温度低于或高于 LCST，还可以有效地萃取三种生物大分子。在 15°C 时，涂层对亲水性生物大分子的吸附容量是 50°C 时的 1.5-2 倍，而在 50°C 时，涂层对 BSA 的吸附容量约为 15°C 时的 3 倍。这种方法对于 SPME 具有很大的应用前景，因为它为各种应用提供了一种简单的策略来制备双功能涂层。
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