Wang Xiao-Hui, Wei Xiao-Fei, Liu Jin-Hua, Yang Wei, Liu Yuan-An, Cheng Kun, He Xiao-Ying, Fu Xiu-Li, Zhang Yu, Zhang Hong-Xin
Beijing Key Laboratory of Work Safety Intelligent Monitoring, School of Electronic Engineering, Beijing University of Posts and Telecommunications, Haidian District, Beijing, 100876, People's Republic of China.
Department of Pulmonary and Critical Care Medicine, the Third Hospital of Changsha, Changsha, 410015, People's Republic of China.
Methods Appl Fluoresc. 2021 Feb 13;9(2):025003. doi: 10.1088/2050-6120/abe219.
A dual-functional nanosysterm is developed by means of Chlorin e6 (Ce6) as photosensitizer and 1,3-Diphenylisobenzofuran (DPBF) as fluorescent singlet oxygen (O) probe. Under 660 nm laser irradiation, Ce6 exhibites efficient O generation, and subsequently the production of O is assessed by the ratiometric fluorescence of PFO and DPBF under one-photon and two-photon excitation mode. The nanoparticles with excellent biocompatibility can be internalized into Hela cells and applied for tumor treatment. For intracellular PDT, the nanoparticles perform a high phototoxicity, while the PDT proccess can be evaluated in time by monitoring fluorescence signals of DPBF. This theranostic nanosysterm provides a facile strategy to fabricate O-detection PDT, which can realize accurate and efficient photodynamic therapy based on singlet oxygen detection.
以二氢卟吩e6（Ce6）作为光敏剂、1,3-二苯基异苯并呋喃（DPBF）作为荧光单线态氧（O）探针，开发了一种双功能纳米系统。在660 nm激光照射下，Ce6表现出高效的单线态氧生成，随后通过在单光子和双光子激发模式下PFO和DPBF的比率荧光来评估单线态氧的产生。具有优异生物相容性的纳米颗粒能够内化进入Hela细胞并应用于肿瘤治疗。对于细胞内光动力疗法（PDT），纳米颗粒表现出高光毒性，同时可以通过监测DPBF的荧光信号及时评估PDT过程。这种诊疗纳米系统提供了一种简便的策略来构建单线态氧检测的光动力疗法，其能够基于单线态氧检测实现准确且高效的光动力治疗。
