Department of Pharmacy, Birla Institute of Technology & Science-Pilani, Hyderabad Campus, Jawahar Nagar, Medchal, Hyderabad 500078, Telangana, India.
Nanomedicine (Lond). 2019 Apr;14(7):819-834. doi: 10.2217/nnm-2018-0255. Epub 2019 Mar 15.
In this study, we developed a chlorin e6-conjugated methoxy-poly(ethylene glycol)-poly(d,l-lactide) (mPEG-PLA-Ce6) amphiphilic polymer, which self-assembled to form stable nanoparticles.
MATERIALS & METHODS: The nanoparticles were characterized for particle size, ζ-potential and singlet oxygen (O) generation. Cellular internalization and phototoxicity were investigated against monolayer and 3D spheroids of human lung adenocarcinoma cells (A549).
RESULTS & CONCLUSION: mPEG-PLA-Ce6 exhibited a size of 149.72 ± 3.51 nm and ζ-potential of -24.82 ± 2.94 mV. The O generation by mPEG-PLA-Ce6 in water was considerably higher than free chlorin e6. The nanoparticles showed enhanced cellular internalization and phototoxicity in monolayer and 3D spheroids. The developed mPEG-PLA-Ce6 has potential application as a nanocarrier of chlorin e6 for photodynamic therapy of solid tumors.
本研究制备了一种喜树碱偶联甲氧基聚乙二醇-聚（丙交酯-乙交酯）（mPEG-PLA-Ce6）两亲性聚合物，其自组装形成稳定的纳米粒子。
对纳米粒子的粒径、ζ-电位和单线态氧（O）生成进行了表征。考察了其对单层和人肺腺癌细胞（A549）3D 球体的细胞内化和光毒性。
mPEG-PLA-Ce6 的粒径为 149.72 ± 3.51nm，ζ-电位为-24.82 ± 2.94mV。mPEG-PLA-Ce6 在水中产生的 O 生成量明显高于游离喜树碱。纳米粒子在单层和 3D 球体中表现出增强的细胞内化和光毒性。所开发的 mPEG-PLA-Ce6 有望作为喜树碱的纳米载体，用于治疗实体瘤的光动力疗法。
