Nasr M L, Goldman M, Klein A K, Dacre J C
Laboratory for Energy-Related Health Research, University of California, Davis 95616.
Mutat Res. 1988 Apr;204(4):649-54. doi: 10.1016/0165-1218(88)90068-7.
Cultured Chinese hamster ovary (CHO) cells were exposed to two neurotoxic organophosphates, either sarin (GBI, GBII) at 1.4 X 10(-3) M or soman (GD) at 1.1 and 2.2 X 10(-3) M for 1 h, grown and their metaphase chromosomes scored for sister-chromatid exchanges (SCE). No cytotoxicity was seen with either agent at any dose level tested. Since histograms of SCE per cell showed that they were non-symmetrically arrayed around the mean, the number of SCEs were analyzed by using the nonparametric tests, Mann-Whitney and Kruskall-Wallis. Agents GBI and GBII did not show any significant increase in SCE over baseline. On the other hand, GD demonstrated a statistically significant increase in SCE with and without metabolic activation. Ethyl methanesulfonate (EMS) alone at 5 X 10(-3) M and cyclophosphamide (CP) at 10(-4) M in the presence of rat microsomes (S9) induced a 3- and 8-fold increase in SCE per cell, respectively.
将培养的中国仓鼠卵巢（CHO）细胞暴露于两种神经毒性有机磷酸酯中，分别是浓度为1.4×10⁻³ M的沙林（GBI、GBII）或浓度为1.1×10⁻³ M和2.2×10⁻³ M的梭曼（GD），处理1小时，然后使其生长，并对中期染色体进行姐妹染色单体交换（SCE）评分。在所测试的任何剂量水平下，两种试剂均未观察到细胞毒性。由于每个细胞的SCE直方图显示它们围绕平均值呈非对称分布，因此使用非参数检验（曼-惠特尼检验和克鲁斯卡尔-沃利斯检验）分析SCE的数量。GBI和GBII试剂在SCE方面未显示出相对于基线的任何显著增加。另一方面，无论有无代谢活化，GD均显示出SCE在统计学上有显著增加。单独使用浓度为5×10⁻³ M的甲磺酸乙酯（EMS）以及在存在大鼠微粒体（S9）的情况下使用浓度为10⁻⁴ M的环磷酰胺（CP），分别使每个细胞的SCE增加了3倍和8倍。
