Université Côte d'Azur, CNRS, Inserm, iBV, France
Université Côte d'Azur, CNRS, Inserm, iBV, France.
Development. 2021 Feb 26;148(4):dev195511. doi: 10.1242/dev.195511.
Basement membranes (BM) are extracellular matrices assembled into complex and highly organized networks essential for organ morphogenesis and function. However, little is known about the tissue origin of BM components and their dynamics Here, we unravel the assembly and role of the BM main component, Collagen type IV (ColIV), in ovarian stalk morphogenesis. Stalks are short strings of cells assembled through cell intercalation that link adjacent follicles and maintain ovarian integrity. We show that stalk ColIV has multiple origins and is assembled following a regulated pattern leading to a unique BM organisation. Absence of ColIV leads to follicle fusion, as observed upon ablation of stalk cells. ColIV and integrins are both required to trigger cell intercalation and maintain mechanically strong cell-cell attachment within the stalk. These results show how the dynamic assembly of a mosaic BM controls complex tissue morphogenesis and integrity.
基底膜（BM）是组装成复杂而高度有序的网络的细胞外基质，对于器官形态发生和功能至关重要。然而，关于 BM 成分的组织来源及其动态变化知之甚少。在这里，我们揭示了基底膜主要成分 IV 型胶原（ColIV）在卵巢柄形态发生中的组装和作用。卵巢柄是通过细胞插入组装成的短串细胞，连接相邻的卵泡并维持卵巢的完整性。我们表明，柄部 ColIV 具有多种起源，并按照一种受调控的模式组装，导致独特的 BM 组织。ColIV 的缺失会导致卵泡融合，如在卵巢柄细胞消融时观察到的那样。ColIV 和整合素都需要触发细胞插入，并在卵巢柄内保持机械上牢固的细胞-细胞附着。这些结果表明，镶嵌基底膜的动态组装如何控制复杂的组织形态发生和完整性。
