Metha B A, Advani S H, Nadkarni S J
Cancer Research Institute, Tata Memorial Centre, Parel, Bombay, India.
Neoplasma. 1988;35(1):61-8.
Natural killer (NK) activity and antibody-dependent cellular cytotoxicity (ADCC) of peripheral blood lymphocytes from untreated non-Hodgkin's lymphoma patients were found to be depressed, when tested against the human erythroblastoid cell-line K562. The percentage of active killer (AK) cells and that of target binding cells (TBC) of the patients were also inhibited. Treatment of the patients lymphocytes with interferon (IFN) caused an augmentation in their NK activity which was comparable with that seen in the controls. Lymphocytes from some of the patients and controls were cocultured with K562 cells for production of natural killer cytotoxic factors (NKCF). The NKCF released by the patients lymphocytes showed a reduced lytic activity against K562 target cells. The depression in all the activities reported here showed a correlation with the clinical status of the patients except in the case of ADCC. These results indicate that further characterization of the properties of NKCF will contribute the understanding of the mechanism of NK cytotoxicity.
当用人类成红细胞样细胞系K562进行检测时，发现未经治疗的非霍奇金淋巴瘤患者外周血淋巴细胞的自然杀伤（NK）活性和抗体依赖性细胞毒性（ADCC）降低。患者的活性杀伤（AK）细胞百分比和靶标结合细胞（TBC）百分比也受到抑制。用干扰素（IFN）处理患者的淋巴细胞会使其NK活性增强，这与在对照组中观察到的情况相当。将一些患者和对照组的淋巴细胞与K562细胞共培养以产生自然杀伤细胞毒性因子（NKCF）。患者淋巴细胞释放的NKCF对K562靶细胞的裂解活性降低。除ADCC外，此处报道的所有活性降低均与患者的临床状况相关。这些结果表明，进一步表征NKCF的特性将有助于理解NK细胞毒性的机制。
