[Differential bioinformational model for diagnostics of inflammatory and tumor diseases of the parotid salivary gland].

THE AIM

Of the work was to develop a diagnostic algorithm for the differentiation of chronic inflammatory, benign and malignant processes in the parotid salivary gland (PSG) by the ratio of pro- and anti-inflammatory cytokines in the oral fluid.

MATERIALS AND METHODS

The epidemiological group of patients with cancer of the parotid salivary gland included 140 people from the oncological register of the Rostov region with the date of diagnosis, from 1969 to 2020. The clinical part of the work was performed on 70 patients of both sexes aged 50 to 80 years: 15 patients with chronic nonspecific parenchymal sialadenitis of the PSG (ICD K11.2) (group 1), 19 patients with pleomorphic adenoma of the PSG (ICD D11.0) (2 group), 20 patients with cancer of the PSG (ICD C07) (group 3) and 16 healthy individuals without pathology of the oral cavity (control group). The concentration of interleukin-6 (IL-6) and interleukin-10 (IL-10) was determined in the oral fluid by enzyme immunoassay.

RESULTS

It was found that in 58.5% of cases at the initial examination of patients with PSG cancer referred to a tertiary care hospital an erroneous opinion was formed about the inflammatory origin of the process. In inflammatory and tumor lesions of the PSG multidirectional differences are noted in the ratio between the concentrations of pro- and anti-inflammatory mediators in the oral fluid. In chronic sialadenitis of PSG in the oral fluid a moderate increase in the levels of IL-6 and IL-10 occurs, in the presence of adenoma of PSG, the concentration of IL-6 does not change while IL-10 increases threefold, and there is a sharp and unidirectional increase in the concentration of cytokines of the opposite groups in case of a malignant lesion of PSG.

CONCLUSION

Comparison of the concentration of IL-6 and IL-10 in saliva and their ratio defined by the developed discriminant models helps to make an individual diagnostic decision in a specific clinical situation.