Cahill A L, Applebaum R, Perlman R L
Department of Pediatrics, University of Chicago, IL 60637.
Neurosci Lett. 1988 Feb 3;84(3):345-50. doi: 10.1016/0304-3940(88)90533-2.
Elongation factor 2 (EF-2) and its associated kinase, Ca2+/calmodulin-dependent protein kinase III, have recently been identified as a major protein phosphorylation system in mammalian tissues. We have measured the phosphorylation of EF-2 in 32P-labeled superior cervical ganglia. Phosphorylation of EF-2 was increased by preganglionic stimulation or by treatment of the ganglion with dimethylphenylpiperazinium or veratridine. Increases in EF-2 phosphorylation presumably reflect the activation of Ca2+/calmodulin-dependent protein kinase III by nicotinic stimulation and depolarization. The phosphorylation of EF-2 may help to coordinate neuronal protein synthesis with neuronal activity.
延伸因子2（EF-2）及其相关激酶，即钙调蛋白依赖性蛋白激酶III，最近已被确定为哺乳动物组织中的主要蛋白质磷酸化系统。我们测量了32P标记的颈上神经节中EF-2的磷酸化情况。节前刺激或用二甲基苯基哌嗪或藜芦碱处理神经节均可增加EF-2的磷酸化。EF-2磷酸化的增加可能反映了烟碱刺激和去极化对钙调蛋白依赖性蛋白激酶III的激活作用。EF-2的磷酸化可能有助于使神经元蛋白质合成与神经元活动相协调。
