School of Radiation Medicine and Protection and School for Radiological and Interdisciplinary Sciences (RAD-X), Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, Medical College of Soochow University, Suzhou, Jiangsu, 215123, China; Yunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan, 650500, China; Key Laboratory for Developmental Genes and Human Disease, Ministry of Education, School of Life Sciences and Technology, Southeast University, Nanjing, 210096, China; Co-innovation Center of Neuroregeneration, Nantong University, Nantong, 226001, China.
Key Laboratory for Developmental Genes and Human Disease, Ministry of Education, School of Life Sciences and Technology, Southeast University, Nanjing, 210096, China.
Colloids Surf B Biointerfaces. 2021 Apr;200:111590. doi: 10.1016/j.colsurfb.2021.111590. Epub 2021 Jan 27.
Neural stem cell (NSC)-based therapy is a promising candidate for treating neurodegenerative diseases and the preclinical researches call an urgent need for regulating the growth and differentiation of such cells. The recognition that three-dimensional culture has the potential to be a biologically significant system has stimulated an extraordinary impetus for scientific researches in tissue engineering and regenerative medicine. Here, A novel scaffold for culturing NSCs, three-dimensional bacterial cellulose-graphene foam (3D-BC/G), which was prepared via in situ bacterial cellulose interfacial polymerization on the skeleton surface of porous graphene foam has been reported. 3D-BC/G not only supports NSC growth and adhesion, but also maintains NSC stemness and enhances their proliferative capacity. Further phenotypic analysis indicated that 3D-BC/G induces NSCs to selectively differentiate into neurons, forming a neural network in a short amount of time. The scaffold has good biocompatibility with primary cortical neurons enhancing the neuronal network activities. To explore the underlying mechanisms, RNA-Seq analysis to identify genes and signaling pathways was performed and it suggests that 3D-BC/G offers a more promising three-dimensional conductive substrate for NSC research and neural tissue engineering, and the repertoire of gene expression serves as a basis for further studies to better understand NSC biology.
神经干细胞（NSC）为基础的治疗是一种很有前途的候选治疗神经退行性疾病和临床前研究呼吁迫切需要调节细胞的生长和分化。承认三维培养有可能成为一个有生物学意义的系统刺激了组织工程和再生医学科学研究的非凡动力。在这里，一种用于培养 NSC 的新型支架，即三维细菌纤维素-石墨烯泡沫（3D-BC/G），通过在多孔石墨烯泡沫骨架表面的界面聚合原位细菌纤维素制备。3D-BC/G 不仅支持 NSC 的生长和粘附，而且保持 NSC 干性并增强其增殖能力。进一步的表型分析表明，3D-BC/G 诱导 NSC 选择性地分化为神经元，在短时间内形成神经网络。该支架与原代皮质神经元具有良好的生物相容性，增强了神经元网络的活性。为了探讨潜在的机制，进行了 RNA-Seq 分析以鉴定基因和信号通路，表明 3D-BC/G 为 NSC 研究和神经组织工程提供了一种更有前途的三维导电基质，基因表达谱为进一步研究 NSC 生物学提供了基础。
