Lin Zhongyuan, Wang Yimin, Lin Shiqing, Liu Decheng, Mo Guohui, Zhang Hui, Dou Yunling
Department of Anesthesiology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510000, Guangdong, China.
Department of Anesthesiology, Guangdong Second Provincial General Hospital, Guangzhou, 510000, Guangdong, China.
BMC Gastroenterol. 2021 Feb 2;21(1):48. doi: 10.1186/s12876-021-01626-7.
Irritable bowel syndrome (IBS) is the most common functional gastrointestinal disease characterized by chronic abdominal discomfort and pain. The mechanisms of abdominal pain, as a relevant symptom, in IBS are still unclear. We aimed to explore the key genes and neurobiological changes specially involved in abdominal pain in IBS.
Gene expression data (GSE36701) was downloaded from Gene Expression Omnibus database. Fifty-three rectal mucosa samples from 27 irritable bowel syndrome with diarrhea (IBS-D) patients and 40 samples from 21 healthy volunteers as controls were included. Differentially expressed genes (DEGs) between two groups were identified using the GEO2R online tool. Functional enrichment analysis of DEGs was performed on the DAVID database. Then a protein-protein interaction network was constructed and visualized using STRING database and Cytoscape.
The microarray analysis demonstrated a subset of genes (CCKBR, CCL13, ACPP, BDKRB2, GRPR, SLC1A2, NPFF, P2RX4, TRPA1, CCKBR, TLX2, MRGPRX3, PAX2, CXCR1) specially involved in pain transmission. Among these genes, we identified GRPR, NPFF and TRPA1 genes as potential biomarkers for irritating abdominal pain of IBS patients.
Overexpression of certain pain-related genes (GRPR, NPFF and TRPA1) may contribute to chronic visceral hypersensitivity, therefore be partly responsible for recurrent abdominal pain or discomfort in IBS patients. Several synapses modification and biological process of psychological distress may be risk factors of IBS.
肠易激综合征（IBS）是最常见的功能性胃肠疾病，其特征为慢性腹部不适和疼痛。IBS中作为相关症状的腹痛机制仍不清楚。我们旨在探索IBS中特别涉及腹痛的关键基因和神经生物学变化。
从基因表达综合数据库下载基因表达数据（GSE36701）。纳入27例腹泻型肠易激综合征（IBS-D）患者的53份直肠黏膜样本和21名健康志愿者作为对照的40份样本。使用GEO2R在线工具鉴定两组之间的差异表达基因（DEG）。在DAVID数据库上对DEG进行功能富集分析。然后使用STRING数据库和Cytoscape构建并可视化蛋白质-蛋白质相互作用网络。
微阵列分析显示了一组特别参与疼痛传递的基因（CCKBR、CCL13、ACPP、BDKRB2、GRPR、SLC1A2、NPFF、P2RX4、TRPA1、CCKBR、TLX2、MRGPRX3、PAX2、CXCR1）。在这些基因中，我们将GRPR、NPFF和TRPA1基因鉴定为IBS患者刺激性腹痛的潜在生物标志物。
某些疼痛相关基因（GRPR、NPFF和TRPA1）的过表达可能导致慢性内脏超敏反应，因此部分导致IBS患者反复出现腹痛或不适。几种突触修饰和心理困扰的生物学过程可能是IBS的危险因素。
