Lago-Sampedro Ana, Ho-Plagaro Ailec, Garcia-Serrano Sara, Santiago-Fernandez Concepción, Rodríguez-Díaz Cristina, Lopez-Gómez Carlos, Martín-Reyes Flores, Ruiz-Aldea Gonzalo, Alcaín-Martínez Guillermo, Gonzalo Montserrat, Montiel-Casado Custodia, Fernández José R, García-Fuentes Eduardo, Rodríguez-Pacheco Francisca
Unidad de Gestión Clínica de Endocrinología y Nutrición, Hospital Regional Universitario, Instituto de Investigación Biomédica de Málaga-IBIMA, Málaga, Spain.
Unidad de Gestión Clínica de Aparato Digestivo, Hospital Universitario Virgen de la Victoria, Instituto de Investigación Biomédica de Málaga-IBIMA, Málaga, Spain.
Clin Nutr. 2021 Jun;40(6):4324-4333. doi: 10.1016/j.clnu.2021.01.015. Epub 2021 Jan 22.
BACKGROUND & AIMS: We investigated whether oleic acid (OA), one of the main components of the Mediterranean diet, participates in the regulation of the intestinal circadian rhythm in patients with morbid obesity.
Stomach and jejunum explants from patients with morbid obesity were incubated with oleic acid to analyze the regulation of clock genes.
Stomach explants showed an altered circadian rhythm in CLOCK, BMAL1, REVERBα, CRY1, and CRY2, and an absence in PER1, PER2, PER3 and ghrelin (p > 0.05). OA led to the emergence of rhythmicity in PER1, PER2, PER3 and ghrelin (p < 0.05). Jejunum explants showed an altered circadian rhythm in CLOCK, BMAL1, PER1 and PER3, and an absence in PER2, REVERBα, CRY1, CRY2 and GLP1 (p > 0.05). OA led to the emergence of rhythmicity in PER2, REVERBα, CRY1 and GLP1 (p < 0.05), but not in CRY2 (p > 0.05). OA restored the rhythmicity of acrophase and increased the amplitude for most of the genes studied in stomach and jejunum explants. OA placed PER1, PER2, PER3, REVERBα, CRY1 and CRY2 in antiphase with regard to CLOCK and BMAL1.
There is an alteration in circadian rhythm in stomach and jejunum explants in morbid obesity. OA restored the rhythmicity of the genes related with circadian rhythm, ghrelin and GLP1, although with slight differences between tissues, which could determine a different behaviour of the explants from jejunum and stomach in obesity.
我们研究了地中海饮食的主要成分之一油酸（OA）是否参与病态肥胖患者肠道昼夜节律的调节。
将病态肥胖患者的胃和空肠外植体与油酸一起孵育，以分析生物钟基因的调节情况。
胃外植体在CLOCK、BMAL1、REVERBα、CRY1和CRY2中显示出昼夜节律改变，而在PER1、PER2、PER3和胃泌素中无昼夜节律（p>0.05）。油酸导致PER1、PER2、PER3和胃泌素出现节律性（p<0.05）。空肠外植体在CLOCK、BMAL1、PER1和PER3中显示出昼夜节律改变，而在PER2、REVERBα、CRY1、CRY2和胰高血糖素样肽-1（GLP1）中无昼夜节律（p>0.05）。油酸导致PER2、REVERBα、CRY1和GLP1出现节律性（p<0.05），但CRY2未出现（p>0.05）。油酸恢复了胃和空肠外植体中大多数研究基因的峰相位节律性并增加了振幅。油酸使PER1、PER2、PER3、REVERBα、CRY1和CRY2与CLOCK和BMAL1呈反相。
病态肥胖患者的胃和空肠外植体存在昼夜节律改变。油酸恢复了与昼夜节律、胃泌素和GLP1相关基因的节律性，尽管组织间存在细微差异，这可能决定了肥胖时空肠和胃外植体的不同行为。
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