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分离流程：用低代次菌株进行实验性感染导致的蜜蜂死亡率高于PRA - 403参考菌株。

Workflow of isolation: Experimental infection with a low-passage strain causes higher honeybee mortality rates than the PRA-403 reference strain.

作者信息

Buendía-Abad María, Higes Mariano, Martín-Hernández Raquel, Barrios Laura, Meana Aranzazu, Fernández Fernández Alberto, Osuna Antonio, De Pablos Luis Miguel

机构信息

IRIAF - Regional Institute for Agrifood and Forestry Research and Development, Laboratory of Bee Pathology, Center for Beekeeping and Agro-environmental Research (CIAPA), Community of Castilla-La Mancha, Marchamalo, Spain.

Institute of Human Resources for Science and Technology (INCRECYT-FEDER), Science and Technology Park Foundation from Castilla - La Mancha, Albacete, Spain.

出版信息

Int J Parasitol Parasites Wildl. 2020 Dec 31;14:68-74. doi: 10.1016/j.ijppaw.2020.12.003. eCollection 2021 Apr.

DOI:10.1016/j.ijppaw.2020.12.003
PMID:33532238
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7829110/
Abstract

The impact of trypanosomatid parasites on honeybee health may represent a major threat to bee colonies worldwide. However, few axenic isolates have been generated to date and with no details on cell culture passages, a parameter that could influence parasite virulence. To address this question, a trypanosomatid isolation protocol was developed and a new strain was obtained, named C1. Using experimental infection of worker honeybees, we compared the virulence and mortality rates of the ATCC PRA-403 reference strain and C1 strain, the latter showing higher virulence from 10 days post-infection onward. This study highlights the impact of cell culture passages on the pathogenicity of in honeybees, providing new evidence of its negative effects on honeybee health.

摘要

锥虫寄生虫对蜜蜂健康的影响可能是全球蜂群面临的重大威胁。然而，迄今为止几乎没有产生无菌分离株，并且由于没有关于细胞培养传代的详细信息，而这是一个可能影响寄生虫毒力的参数。为了解决这个问题，我们开发了一种锥虫分离方案，并获得了一个新菌株，命名为C1。通过对工蜂进行实验性感染，我们比较了ATCC PRA - 403参考菌株和C1菌株的毒力和死亡率，结果显示C1菌株在感染后10天起毒力更高。这项研究突出了细胞培养传代对蜜蜂体内寄生虫致病性的影响，为其对蜜蜂健康的负面影响提供了新证据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c6f/7829110/01345c83ea2a/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c6f/7829110/8653c5a4f086/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c6f/7829110/e186c6e0d477/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c6f/7829110/01345c83ea2a/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c6f/7829110/8653c5a4f086/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c6f/7829110/e186c6e0d477/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c6f/7829110/01345c83ea2a/gr2.jpg

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