双 GFP-Tocky 报告小鼠在流式细胞术中研究 T 细胞受体信号转导的应用。
Application of dual -GFP -Tocky reporter mice to study T cell receptor signaling by flow cytometry.
机构信息
Institute of Immunology and Immunotherapy, College of Medical and Dental Sciences, University of Birmingham, Birmingham B15 2TT, UK.
Department of Life Sciences, Imperial College London, London SW7 2AZ, UK.
出版信息
STAR Protoc. 2021 Jan 21;2(1):100284. doi: 10.1016/j.xpro.2020.100284. eCollection 2021 Mar 19.
This protocol uses -GFP -Tocky mice to study T cell receptor (TCR) signaling using flow cytometry. It identifies the optimal mouse transgenic status and fluorochromes compatible with the dual reporter. This protocol has applications in TCR signaling, and we outline how to obtain high-quality datasets. It is not compatible with cellular fixation, and cells should be analyzed immediately after staining. For complete details on the use and execution of this protocol, please refer to Jennings et al., 2020.
本方案使用 -GFP -Tocky 小鼠通过流式细胞术研究 T 细胞受体（TCR）信号。它确定了与双报告基因兼容的最佳小鼠转基因状态和荧光染料。本方案适用于 TCR 信号研究，我们概述了如何获得高质量数据集。它与细胞固定不兼容，细胞应在染色后立即进行分析。如需了解本方案使用和实施的详细信息，请参考 Jennings 等人，2020。
Zotero 插件