Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, Mexico.
Posgrado en Ciencias Bioquímicas, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, Mexico.
J Cell Physiol. 2021 Sep;236(9):6643-6656. doi: 10.1002/jcp.30308. Epub 2021 Feb 2.
Aberrant expression of CD43 in malignant tumors of nonhematopoietic origin such as those from lung, cervix, colon, and breast has been shown to correlate with poor prognosis, providing tumor cells with enhanced motility, anchorage-independent growth, and in vivo tumor size, while protecting the cells of NK lysis and apoptosis. To further characterize the role of CD43 in cell transformation, we tested whether interfering its expression modified the capacity of the A549 non-small cell lung cancer cells to secrete molecules contributing to malignancy. The proteomic analysis of the secretome of serum-starved A549 cells revealed that cells expressing normal levels of CD43 released significantly high levels of molecules involved in extracellular matrix organization, angiogenesis, platelet degranulation, collagen degradation, and inflammation, as compared to CD43 RNAi cells. This data reveals a novel and unexpected role for CD43 in lung cancer development, mainly in remodeling the tumor microenvironment.
异常表达的 CD43 在非造血来源的恶性肿瘤中，如肺癌、宫颈癌、结肠癌和乳腺癌，与预后不良相关，为肿瘤细胞提供了更强的迁移能力、锚定独立生长和体内肿瘤大小，同时保护细胞免受 NK 溶解和凋亡。为了进一步研究 CD43 在细胞转化中的作用，我们检测了干扰其表达是否会改变 A549 非小细胞肺癌细胞分泌参与恶性肿瘤的分子的能力。血清饥饿 A549 细胞的分泌组的蛋白质组学分析表明，与 CD43 RNAi 细胞相比，表达正常水平 CD43 的细胞释放了大量参与细胞外基质组织、血管生成、血小板脱颗粒、胶原降解和炎症的分子。这些数据揭示了 CD43 在肺癌发展中的一个新的、意想不到的作用，主要是在重塑肿瘤微环境。
